紫杉醇与多烯紫杉醇对小鼠卵巢储备能力的影响

Effects of Paclitaxel and Docetaxel on Ovarian Reserve Capacity in Mice

目的:探索多次重复小剂量紫杉醇与多烯紫杉醇对小鼠卵巢储备功能的影响。方法:30只昆明雌性小鼠随机分组,即对照组(生理盐水0.2 ml/d×20 d)、紫杉醇组[紫杉醇注射液6.0 mg/(kg·d)×20 d]、多烯紫杉醇组[多西他赛注射液1.5 mg/(kg·d)×13 d],每组10只,用药期间每日观察小鼠的一般情况、动情周期,用药结束后观察小鼠血清FSH、E2水平、卵巢质量、形态结构和各级卵泡数的变化。结果:用药结束后紫杉醇组FSH水平(54.59±12.73 pg/ml)高于对照组(31.85±9.82 pg/ml),E2水平(27.39±9.36 pg/ml)低于对照组(55.26±13.41 pg/ml),差异有统计学意义(P<0.05);而多烯紫杉醇组FSH(33.27±5.91 pg/ml)、E2(49.56±3.57 pg/ml)变化较对照组不明显,差异无统计学意义(P>0.05);紫杉醇组FSH、E2水平变化较多烯紫杉醇组差异有统计学意义(P<0.05)。紫杉醇组小鼠的总卵泡数(598±159个)和卵巢质量(7±2 mg)与对照组小鼠(1 243±137个,27±6 mg)比较,差异均有统计学意义(P<0.05);多烯紫杉醇组小鼠的总卵泡数(1 143±130个)和卵巢质量(22±5 mg)与对照组小鼠比较,差异均无统计学意义(P>0.05);小鼠的总卵泡数和卵巢质量组间差异均有统计学意义(P<0.05)。结论:多次重复小剂量紫杉醇与多烯紫杉醇对卵巢储备功能均有不同程度的影响,其中紫杉醇影响更为严重。

Objective： To study the effect of repeated small doses of paclitaxel and docetaxel on the ovarian reserve. Methods： Thirty female KM mice were randomly divided into three groups： control group （NS 0.2 ml/d, for 20 d, i.p）, paclitaxel group [6.0 mg/（kg.d）, for 20 d, i.p], and docetaxel group [1.5 mg/（kg.d）, for 13 d, i.p]. Body weight, estrous cycle daily during treatment, and FSH, E2 levels in serum, ovarian weight, histopathology,the number of follicles after treatment were measured, then those indicators were cpmpared among the three groups. Results： After treatment, there were significant differences between paclitaxel group and control group in serum levels of FSH and E2 （54.59 ±12.73 pg/ml vs 31.85 ± 9.82 pg/ml and 27.39 ± 9.36 pg/ml vs 55.26 ± 13.41 pg/ml）, P〈0.05 for both comparisons. But there were no significant differences of serum FSH （33.27±5.91 pg/ml） and E2 （49.56±3.57 pg/ml） levels in docetaxel group compared with those of control group. There were significant differences between paclitaxel group and docetaxel group in both FSH and E2 levels in serum.There were significant differences between paclitaxel group and control group in two levels as follows： the total number of follicles （598 ± 159 vs 1 243±137） and the weight of ovaries （7 ± 2 mg vs 27 ± 6 mg）. But there were no significant differences in the total number of follicles （1 143 ±130） and the weight of ovaries （22 ± 5 mg） in docetaxel group compared with those of control group. There were significant differences between paclitaxel group and docetaxel group in the total number of follicles and the weight of ovaries. Conclusion： Repeated small doses of paclitaxel and docetaxel impact the ovarian reserve function in different extent, the effect of paclitaxel was more serious.