两种啤酒花浸膏对雄性小鼠生殖毒性的研究

Study on Reproductive Toxicity of Hops Extracts to Male Mice

目的:观察2种不同厂家啤酒花浸膏(hops extract)对雄性小鼠生殖功能的作用。方法:成年小鼠随机分为A组和B组,分别灌胃给予A、B厂家的啤酒花浸膏连续20 d,均设3个剂量亚组:按国家规定的人嗜酒标准的1倍剂量(低剂量组)、10倍剂量(中剂量组)和100倍剂量(高剂量组)给予以玉米油为溶剂的啤酒花浸膏;另设溶剂对照组,每亚组5只动物。观察小鼠附睾尾精子数量、精子存活率、活动精子数、精子畸形率、生精小管直径,测定血清中睾酮(T)、双氢睾酮(DHT)和雌二醇(E2)的含量以及观察肝、脾、肾、睾丸、附睾等器官的病理改变。结果:2种不同啤酒花浸膏灌胃组小鼠的精子畸形率均较对照组高,睾丸的脏器系数均高于对照组,血清T、DHT和E2含量均低于对照组,差异有统计学意义。HE染色结果显示摄入各剂量啤酒花浸膏小鼠对睾丸、附睾组织结构无明显影响。结论:高剂量组的两种不同啤酒花浸膏对小鼠的精子均有致畸作用,并可使血清T、DHT和E2含量降低。

Objective： To observe the effect of two different manufacturers hops extracts on reproductive function of male mice. Methods： Adult mice were fed hops extracts by oral administration for 20 d, and were divided into the following groups, low dose A （0.05 mg/kg）, middle dose A （0.50 mg/kg）, high dose A （5.00 mg/kg） （give hops extract A）, low dose B （0.075 mg/kg）, middle dose B （0.750 mg/kg）, high dose B （7.500 mg/kg） （give hops extract B）. The epididymal sperm count, sperm viability, activities sperm count, sperm deformity rate, seminiferous tubule diameter were observed, serum testosterone （T）, dihydrotestosterone （DHT） and estradiol （E2） concentrations were detected and the pathological examination of the liver, spleen, kidney, testis, epididymis and other organs was performed. Results： The sperm abnormality rate of mice fed on two different hops extracts was higher than that in the control, testis organ coefficient was higher, serum T, DHT and E2 levels were lower than those of the control, and there were no significant toxicity effects on the testis and epididymis lbr each dose group. Conclusion： The two different hops extracts which doses are higher than the alcohol dose have teratogenic effects on mouse sperm and reduce the level of serum T, DHT and E2 at the same time.