细菌非编码小RNA的筛选及鉴定方法

Screening and Identification of Small Non-coding RNAs in Bacteria

细菌非编码小RNA（smallnon．codingRNAs，sRNAs）是一类长度为50～500nt、不编码蛋白质的功能RNA，在应对胁迫、毒力产生和新陈代谢等生命过程中起重要的调控作用。其主要通过碱基配对与靶mRNA发生作用，导致mRNA翻译和稳定性改变，从而在转录后水平调节基因的表达，最终影响细菌各种生命活动。近年来，利用生物信息学和分子生物学技术，已在细菌中筛选并鉴定得到了几百个sRNA。该文对细菌sRNA的筛选和鉴定方法作一简要综述。

Small non-coding RNAs （sRNAs） in bacteria are 50-500 nt small RNAs that do not encode proteins, but act as important regulators in response to stress, virulence and metabolism. Most small RNAs interact with target mRNAs through base-pairing to change their translation and stability so as to affect the activity of bacteria. Recently, hundreds of sRNAs have been explored in bacteria using bioinformatics combined with molecular biology techniques. This review focuses on the screening and identification of sRNAs in bacteria.