新型Micron Ⅳ视网膜影像系统在三种小鼠疾病模型中的应用

Application of Micron Ⅳ retinal imaging system in three different types of mouse models

目的 观察新型MicronⅣ视网膜影像系统检查在3种小鼠疾病模型中的应用效果.方法 健康雄性C57BL/6J小鼠24只.分别建立氧诱导视网膜病变（OIR）模型组、N-甲基-N-亚硝脲（MNU）模型组、N-甲基-D-天冬氨酸（NMDA）模型组.应用MicronⅣ视网膜影像系统对3种模型组小鼠行眼底彩色照相;OIR模型组、MNU模型组小鼠行荧光素眼底血管造影（FFA）、光相干断层扫描（OCT）检查;MNU模型组、NMDA模型组小鼠行视网膜电图（ERG）检查.结果 OIR模型组小鼠眼底视网膜血管高度扩张、纡曲,走形僵直;FFA检查可见形态异常、分布紊乱的新生血管丛,荧光渗漏致玻璃体混浊;OCT检查可见视网膜前纤维血管组织及纡曲扩张血管后方粗大的阴影暗区.MNU模型组小鼠眼底视盘呈蜡黄色萎缩,视网膜无明显色素性改变;FFA检查可见视网膜血管轻微变细;OCT检查可见视网膜厚度和结构变化不明显;ERG检查结果显示,a、b波潜伏期变化不大,建模型后2、3d最大混合反应a波振幅分别为（15.38±4.36）、（13.78±5.52） μV,明显下降.NMDA模型组小鼠眼底视网膜呈苍白色改变,血管痉挛纡曲;ERG检查结果显示,a、b波潜伏期变化不大,建模型12、24 h最大混合反应b波振幅分别为（72.28±7.18）、（65.35±9.18） μV,明显下降.结论 Micron Ⅳ视网膜影像系统能实时、无创观察视网膜结构和功能.

Objective To evaluate Micron Ⅳ retinal imaging system in three mouse models of retinal diseases.Methods Mouse models of oxygen induced retinopathy （OIR） model （OIR group）,N-methyl-N nitrosourea （MNU） model （MNU group） and N-methyl-D-aspartate （NMDA） model （NMDA group） were induced in 24 healthy male C57BL/6J mice.Fundus photograph,fundus fluorescein angiography （FFA） and optical coherence tomography （OCT） and electroretinogram （ERG） were used to evaluate these mice.All the imaging examinations were performed by Micron Ⅳ retinal imaging system.Results OIR mice showed tortuous and dilated retinal vessels in fundus photograph,neovascularization plexus and vascular leakage in FFA,and epiretinal fibrovascular tissue and tortuous expansion vascular vessels in OCT.MNU mice showed wax yellow optic disk without retinal pigmentary changes,slight thinning of retinal blood vessels in FFA,and normal structure and thickness in OCT.The a-wave amplitudes of the maximum mixed response decreased significantly,and were （15.38±4.36） μV and （13.78±5.52） μV at 2 or 3 days of modeling,respectively.NMDA mice showed a pale retina with vasospasm.ERG revealed that there was no obvious change in latency of a-and b-wave,but significantly decreased amplitude of b-wave at 12 hours and 24 hours after modeling with （72.28±7.18） μV and （65.35±9.18） μV,respectively.Conclusion Micron Ⅳ retinal imaging system is a real-time,non-invasive tool to study the retinal structure and function in animal models of retinal diseases.