激光多普勒血流仪监测C57BL/6小鼠永久性大脑中动脉闭塞模型

Monitoring of Permanent Middle Cerebral Artery Occlusion Model in C57BL/6 Mice by Laser Doppler Flowmetry

目的评估激光多普勒血流仪监测C57BL/6小鼠永久性大脑中动脉闭塞(MCAO)模型的可行性。方法 C57BL/6小鼠随机分为2组,假手术组和永久性MCAO组。线栓法制作永久性大脑中动脉闭塞模型,激光多普勒仪实时监测小鼠脑血流,术后神经功能学评分,24 h后2,3,5-三苯基氯化四氮唑染色确定梗死体积。结果 MCAO组术前脑血流(186.78±62.50)PU,栓线插入成功后患侧脑血流降低到(25.80±7.66)PU,降低了90.4%。神经功能学评分在(2.48±0.36)分,脑梗死面积达39.79%。假手术组脑血流在基础值波动,未见降低;神经功能学评分为0分,未见脑梗死病灶。结论激光多普勒血流仪监测是一个很好的确定小鼠脑梗死模型成功与否的筛选方法。

[Abstract] Objective To evaluate the feasibility of monitoring of permanent middle cerebral artery occlusion （MCAO） model in C57BL/6 mice by Laser Doppler flowmetry. Methods C57BL/6 mice were divided into 2 groups randomly： sham group and permanent MCAO group. Permanent MCAO model was established with the method of suture inserted into the internal carotid artery. Sufficiency of MCAO was monitored by Laser Doppler flowmetry during ischemia in mice. The neurological deficit score was assessed and the cerebral infarction size was measured by 2, 3, 5-triphenytetrazolium chloride （TTC） staining technique twenty-four hours after MCAO. Results In the MCAO group, the local blood flow was decreased from the preoperative value of（186.78 ± 62.50） PU to the postoperative value of （25.80 ± 7.66） PU. Cerebral blood flow was reduced by 90.4% during MCAO. The neurological deficit score was 2.48 ± 0.36. The cerebral infarction area accounted for 39.79% by TTC staining. However, the cerebral blood flow fluctuations were not reduced and the neurological deficit score was found normal in the sham group. Furthermore, there was no cerebral infarction lesion in the sham group. Conclusion Monitoring by Laser Doppler flowmetry is efficient for evaluating the success rate of MCAO.