天麻多糖与电针对脑缺血大鼠齿状回BDNF和VEGF表达的影响

Effects of polysaccharide of gastrodia elata blume and electroacupuncture on the expressions of brain derived neurotrophic factor and vascular endothelial growth factor in dentate gyrus of cerebral ischemia rats

目的:观察天麻多糖与电针对脑缺血大鼠齿状回(dentate gyrus,DG)脑源性神经营养因子(brain derived neurotrophic factor,BDNF)和血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法:将40只SD大鼠随机分为正常对照组、模型组、电针组、天麻多糖组和针药结合组,每组8只。以单侧大脑中动脉栓塞法制备脑缺血模型。造模后2周,天麻多糖组和针药结合组大鼠给予天麻多糖100mg/kg灌胃,每天1次,连续2周;电针组和针药结合组大鼠给予"百会"、"足三里"穴电针刺激,持续30min,每天1次,连续2周。采用免疫组织化学染色法结合图像分析检测DG BDNF和VEGF的表达。结果:与正常对照组比较,模型组缺血侧DG BDNF和VEGF阳性表达增加(P<0.05);与模型组比较,电针组、天麻多糖组和针药结合组缺血侧DG BDNF和VEGF阳性表达明显增加(P<0.05);针药结合组阳性表达显著多于电针组或天麻多糖组(P<0.01)。结论:天麻多糖与电针结合可显著增加脑缺血大鼠缺血侧DG BDNF和VEGF的表达,促进内源性神经干细胞激活,且作用优于单用电针或天麻多糖。

AIM：To observe the effects of polysaccharide of gastrodia elata blume（PGB） and electroacupuncture（EA）on the expression of brain derived neurotrophic factor（BDNF）and vascular endothelial growth factor（VEGF）in dentate gyrus（DG）of hippocampus of cerebral ischemia rats.METHODS：A total of 40Sprague-Dawley rats were randomly divided into the normal control group,the model group,the EA group,the PGB group and the EA＋PGB group,8in each group.The model was prepared by middle cerebral artery occlusion（MCAO）.2 week after modeling,PGB at 100 mg/kg was given to rats in the PGB group and the EA＋ PGB group by gastrogavage,once daily,for 2 successive weeks.EA at＂Baihui＂（DU20）and ＂Zusanli＂（ST36）was performed to rats in the EA group and the EA＋PGB group,lasting for 30min,once daily,for 2successive weeks.The expressions of BDNF and VEGF in the DG detected by immunohistochemical assay and image analysis.RESULTS：Compared with the normal control group,the expressions of BDNF and VEGF in the DG of the model group were increased（P〈0.05）.Compared with the model group,the expressions of BDNF and VEGF in the DG of the EA group,the PGB group and the EA＋PGB group were increased significantly（P〈 0.05）.The expressions of BDNF and VEGF obviously were increased in the EA ＋ PGB group than those of the EA group or the PGB group（P〈0.01）.CONCLUSION：EA combined with PGB could significantly upregulat the expressions of BDNF and VEGF in the DG of cerebral ischemia rats,accelerate endogenous neural stem cells in the DG,besides,their effects were significantly higher than those of the PGB group or the EA group.