摘要
目的:研究连翘酯苷对顺铂作用后豚鼠耳蜗c-jun表达的影响。方法:将30只豚鼠随机分为对照组(10只),顺铂组(10只)和连翘酯苷组(10只)。腹腔注射顺铂溶液(8mg/kg),1次/d,连续7d,建立顺铂耳毒性模型;连翘酯苷组在每次注射顺铂溶液30min前腹腔注射连翘酯苷25.0mg/kg/d,连续7d;对照组以生理盐水代替顺铂溶液注射,连续7d。实验动物被处死前,检测其DPOAE幅值变化;采用蛋白质印迹杂交(Western Blotting)检测各组豚鼠耳蜗c-jun蛋白的表达,逆转录聚合酶链反应检测各组豚鼠耳蜗c-jun基因mRNA的表达。结果:顺铂组DPOAE幅值明显低于对照组(P<0.01);相比于顺铂组,连翘酯苷组DPOAE幅值明显升高(P<0.05)。顺铂组豚鼠耳蜗c-jun蛋白与mRNA表达水平均显著高于对照组(P<0.01);相比于顺铂组,连翘酯苷组c-jun蛋白与mRNA表达水平明显降低(P<0.05)。结论:连翘酯苷能够通过降低c-jun的表达防护顺铂所致的耳蜗损伤。
Objective：To study the effect of forsythiaside on the expression of c-jun induced by eisplatin in the cochlea of guinea pig. Method： Thirty guinea pigs were randomly divided into control group （10）, eisplatin group （10） and forsythiaside group （10）. The ototoxicity model was done with intraperitoneal injection of cisplatin solution （8 mg/kg per day） for 7 days. Forsythiaside （25 mg/kg per day） was injected 30 rain before cisplatin solution treated in guinea pigs of forsythiaside group for 7 consecutive days. The saline instead of cisplatin was injected in normal control group. The distortion product otoaeoustic emission （DPOAE） was detected before animals were killed. The expression of c-jun in cochlea of guinea pigs was detected by western blotting. The expression of c-jun mRNA in cochlea of guinea pigs was detected by reverse transcriptase polymerase chain reaction （RT-PCR）. ResuIt：DPOAE amplitudes in cisplatin group was significantly lower than in control group （P〈0.01）. Compared with cisplatin group, DPOAE amplitudes in forsythiaside group was increased significantly （P〈0.05）. The expression of c-jun protein and mRNA were significantly increased in cisplatin group than in control group （P〈 0.01）. Compared with cisplatin group, the expression of c-jun protein and mRNA were significantly decreased in forsythiaside group. Conclusion： Forsythiaside can significantly reduce the side effects induced by cisplatin through down-regulating the expression of c-jun.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2014年第10期731-734,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
