土生空团菌的遗传多样性分析

Genetic diversity analysis of Cenococcum geophilum

为了研究土生空团菌的遗传多样性,对来自中国、美国、瑞士和法国等菌株的rDNA ITS区进行序列分析,利用Popgene32和phylip软件进行数据计算和聚类分析。序列分析表明土生空团菌rDNA ITS区的序列长度为422–447bp,遗传距离在0.000–0.051之间。居群结构和聚类分析结果表明:(1)土生空团菌有一定的遗传多样性,且遗传差异主要来自于居群内;(2)基因流Nm>1,遗传漂变不是导致土生空团菌居群遗传分化的主要因素;(3)土生空团菌的遗传分化受到地理环境的影响,而与宿主来源没有明显相关性。

To analysis the genetic diversity of Cenococcum geophilum, the sequences of rDNA ITS region were aligned among C. geophilum strains from China, USA, Switzerland and France, the data calculation and phylogenetic analyses were performed by Popgene32 and PHYLIP softwares. Sequence analyses showed that the rDNA ITS of C. geophilum ranged from 422 to 447 nucleotides in length, and their genetics distance were from 0.000 to 0.0S1. Population structure and phylogenetic analysis showed that： （1） C. geophilum has certain degree of genetic diversity, and the genetic variation is mainly within populations; （2） Nm〉l, and the genetic drift is not the principal factor that results in genetic variation of C geophilum; （3） The effects of host origin on C. geophilum genetic diversity are not obvious, however, geographic environment influences genetic differentiation of C. geophilum.