荷叶中化学成分的分离与鉴定(Ⅱ)

Isolation and identification of chemical constituents from the leaves of Nelumbo nucifera Gaertn.(Ⅱ)

目的对荷叶中的化学成分进行分离与鉴定。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及制备型反相HPLC法分离纯化,并利用MS、NMR、单晶衍射等方法解析化合物的化学结构。结果从荷叶体积分数为70%的乙醇提取物中分离鉴定了6个化合物,分别鉴定为：黄芩新素Ⅱ（skullcapflavoneⅡ,1）、粘毛黄芩素Ⅲ（viscidulinⅢ,2）、4-methyl-6-phenyl-2H-2-pyranone（3）、牛蒡酚F（lappaol F,4）、黑麦草内酯（loliolide,5）和黄柏酮（obacunone,6）。结论化合物1~6均为首次从莲属植物中分离得到。

Objective To isolate the chemical constituents of the leaves of Nelumbo nucifera Gaertn. and elucidate their structures. Methods The compounds w ere isolated by silica gel,ODS,Sephadex LH-20 column chromatography and RP-HPLC,and their structures w ere identified by MS,NMR and X-ray single crystal diffraction spectroscopies. Results Six compounds w ere isolated from 70% ethanol extract of lotus leaves and identified as skullcapflavoneⅡ（ 1）,viscidulin Ⅲ（ 2）,4-methyl-6-phenyl-2H-2-pyranone（ 3）,lappaol F（ 4）,loliolide（ 5） and obacunone（ 6）,respectively. Conclusions Compounds 1-6 are obtained from Nelumbo Adans. for the first time.