HPLC法同时测定养血清脑胶囊中芍药苷和阿魏酸的含量

Determination of paeoniflorin and ferulic acid in Yangxue Qingnao capsule by HPLC

目的建立同时测定养血清脑胶囊中芍药苷和阿魏酸含量的方法。方法采用HPLC法。色谱柱为Diamonsil C18柱（250 mm×4.6 mm,5μm）,流动相为乙腈-体积分数0.085%磷酸溶液,梯度洗脱,流速为1.0 mL·min-1,检测波长为240 nm,柱温为30℃。结果芍药苷质量浓度在4.96-19.84 mg·L-1内与峰面积呈良好的线性关系（r=0.999 6）,平均回收率为97.1%（RSD=1.9%,n=6）;阿魏酸质量浓度在2.52-10.08 mg·L-1内与峰面积呈良好的线性关系（r=0.999 0）,平均回收率为100.1%（RSD=2.8%,n=6）。结论建立的方法简便、准确、重复性好,可用于养血清脑胶囊的质量控制。

Objective To establish a method for simultaneous determination of contents of peoniflorin and ferulic acid in Yangxue Qingnao capsule（ traditional Chinese medicine）. Methods HPLC w ith Diamonsil C18column（ 250 mm × 4. 6 mm,5 μm） w as used,acetonitrile- 0. 085% phosphoric acid（ gradient elution） w as selected as a mobile phase. The detection w avelength w as set at 240 nm. Flow rate w as 1. 0 mL·min- 1, and column temperature w as 30 ℃. Results The linear range of peoniflorin w as 4. 96-19. 84 mg·L- 1（ r = 0. 999 6）,the average recovery w as 97. 1%（ RSD = 1. 9%,n = 6）. The linear range of ferulic acid w as 2. 52-10. 08 mg·L- 1（ r =0. 999 0）,the average recovery was 100. 1%（ RSD =2. 8%,n =6）. Conclusions The method is simple,accurate and reproducible for simultaneous determination of peoniflorin and ferulic acid. It can be used for the quality control of compound Yangxue Qingnao capsule.