摘要
目的建立同时测定养血清脑胶囊中芍药苷和阿魏酸含量的方法。方法采用HPLC法。色谱柱为Diamonsil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-体积分数0.085%磷酸溶液,梯度洗脱,流速为1.0 mL·min-1,检测波长为240 nm,柱温为30℃。结果芍药苷质量浓度在4.96-19.84 mg·L-1内与峰面积呈良好的线性关系(r=0.999 6),平均回收率为97.1%(RSD=1.9%,n=6);阿魏酸质量浓度在2.52-10.08 mg·L-1内与峰面积呈良好的线性关系(r=0.999 0),平均回收率为100.1%(RSD=2.8%,n=6)。结论建立的方法简便、准确、重复性好,可用于养血清脑胶囊的质量控制。
Objective To establish a method for simultaneous determination of contents of peoniflorin and ferulic acid in Yangxue Qingnao capsule( traditional Chinese medicine). Methods HPLC w ith Diamonsil C18column( 250 mm × 4. 6 mm,5 μm) w as used,acetonitrile- 0. 085% phosphoric acid( gradient elution) w as selected as a mobile phase. The detection w avelength w as set at 240 nm. Flow rate w as 1. 0 mL·min- 1, and column temperature w as 30 ℃. Results The linear range of peoniflorin w as 4. 96-19. 84 mg·L- 1( r = 0. 999 6),the average recovery w as 97. 1%( RSD = 1. 9%,n = 6). The linear range of ferulic acid w as 2. 52-10. 08 mg·L- 1( r =0. 999 0),the average recovery was 100. 1%( RSD =2. 8%,n =6). Conclusions The method is simple,accurate and reproducible for simultaneous determination of peoniflorin and ferulic acid. It can be used for the quality control of compound Yangxue Qingnao capsule.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2014年第5期375-378,383,共5页
Journal of Shenyang Pharmaceutical University
关键词
养血清脑胶囊
芍药苷
阿魏酸
HPLC
含量测定
Yangxue Qingnao capsule
peoniflorin
ferulic acid
HPLC
content determination