miR-26b和miR-224在梅山与杜洛克猪卵泡中的差异表达分析

Differential Expression Analysis of miR-26b and miR-224 in Ovarian Follicles of Meishan and Duroc Sows

为了探讨miRNAs在猪卵泡和繁殖相关组织中的表达,采用荧光定量RT-PCR法和组织表达谱分析法,对miR-26b和miR-224在梅山和杜洛克猪卵泡及其他繁殖相关组织中的表达进行了研究。组织表达谱分析结果表明:miR-26b和miR-224在下丘脑、垂体、子宫、输卵管、卵巢、子宫角、黄体组织中均有表达。RT-PCR结果表明:miR-26b在L卵泡中的表达量梅山猪是杜洛克猪的2倍(P<0.01);而在S卵泡中的表达量杜洛克是梅山猪的10倍(P<0.01);两品种在M1和M2卵泡中的表达量差异不显著。miR-224在S卵泡中的表达量杜洛克是梅山的7.79倍(P<0.01);而在M2卵泡中表达量梅山是杜洛克的1.84倍(P<0.05);两品种在M1和L卵泡中的表达量差异不显著。在猪种内大卵泡L、中等卵泡M2、中等卵泡M1、小卵泡S的相对表达量也有不同程度的差异。此研究表明,miR-26b和miR-224在梅山和杜洛克猪中表达趋势相似,它们的表达差异可能影响卵泡发育和成熟,为深入了解miR-26b和miR-224在卵泡发育中的作用奠定了基础。

To explore the expression of miR-26b and miR-224 in ovarian follicles and reproduction-related tissues of Duroc and Meishan Sows,quantitative Real-time RT-PCR （qRT-PCR） technique was used in this study. The results showed that miR-26b and miR-224 were both expressed in hypothalamus,hypophysis,uterus,cornua uteri,ovary,oviduct and corpus luteum.The qRT-PCR results indicated that miR-26b and miR-224 were differentially expressed in follicles of different size and breed.For miR-26b,the relative expression amount in follicle L of Meishan was twice of that in Duroc, however,the expression amount in follicle S Duroc was ten times of that in Meishan.For miR-224,the relative expression amount in follicle S Duroc was 7.79 times of that in Meishan, but the expression amount in follicle M2 Meishan was 1.84 times of that in Duroc.These different expression patterns of miR-26b and miR-224 in the follicle of Meishan and Duroc maybe affect the development and maturation of follicles. Our research will be useful for further study on the molecular mechanism of ovulation regulatory network.