杂交油葵A15种子纯度的RAPD鉴定

Identification of Seed Purity of Oilseed Sunflower (Helianthus annuus L.) Hybrid A15 with RAPD

从杂交油葵Ａ１５及其亲本中提取基因组ＤＮＡ，用１８０个ＲＡＰＤ随机引物进行扩增，从中筛 选出３个可将亲本和子代区分开的引物ＯＰＤ０９、ＯＰＤ１２和ＯＰＫ１２。ＯＰＤ０９产生亲本互补的特征带 ＯＰＤ０９－１４７０ｂｐ、ＯＰＤ０９－８７０ｂｐ；ＯＰＤ１２产生母本特征带ＯＰＤ１２－１２３０ｂｐ，ＯＰＫ１２产生父本特征带 ＯＰＫ１２－１５４０ｂｐ、ＯＰＫ１２－９４０ｂｐ，上述谱带均在子代中出现。以单引物（ＯＰＤ０９）和双引物（ＯＰＤ１２和 ＯＰＫ１２）产生的这两组特征谱带作为分子标记分别对杂交油葵种子纯度进行鉴定得到了一致的结果， 并与大田纯度检测结果相一致。用ＲＡＰＤ鉴定杂交油葵种子纯度是可行的。

The genomic DNAs extracted from young leaves of oilseed sunflower hybrid A15 and its parents were analyzed using polymerase chain reaction (PCR) with arbitrary 10-mer primers to generate random amplified polymorphic DNA (RAPD) markers. Among 180 RAPD primers, three primers, OPD09, OPD12, OPK12, which could generate differential RAPD profiles among A15 and its parents, were screened. Primer OPD09 could generate parental complementary characteristic bands: OPD09-1470bp, OPD09-870bp; primer OPD12 could generate maternal characteristic band OPD12-1230bp ; primer OPK12 could generate paternal characteristic bands OPK12-1540bp and OPK12-940bp. All these characteristic bands screened were found in A15 hybrids. These bands can be used as RAPD markers to identify seed purity of A15. It is viable to identify oilseed sunflower hybrids seed purity with RAPD.