活检时期对非优质卵裂胚发育潜力及冻存效果的影响

Effect of biopsy stage on developmental potential and cryopreservation effectiveness of poor quality cleavage embryos

目的:探讨非优质卵裂期胚胎的活检价值及最佳活检时期。方法:选择体外受精-胚胎移植周期患者废弃的受精后第3天非优质胚胎,随机分为卵裂期活检组和囊胚期活检组,卵裂期活检组胚胎于受精后第3天活检1个卵裂球,而囊胚期活检组胚胎延长培养至囊胚期再行滋养层细胞活检,选择两组形成的可冻囊胚进行玻璃化冷冻,比较两组胚胎的囊胚形成情况及玻璃化冻融结局。结果:卵裂期活检组的优质囊胚形成率显著低于囊胚期活检组(1.64%vs14.75%,P=0.017),两组形成的囊胚玻璃化解冻复苏率无统计学差异。结论:非优质卵裂期胚胎具有一定的活检价值,囊胚期是其活检的最佳时期。

Objective： To explore biopsy value of poor quality cleavage embryos and the optimal embryo stage for biopsy. Methods： The discard poor quality embryos on the third day after fertilization from IVF /ICSI- ET cycles were randomly divided into cleavage stage biopsy group and blastocyst biopsy group,biopsy of one blastomere was performed on embryos in cleavage stage biopsy group,and the trophectoderm biopsy was performed during blastula stage in blastocysts biopsy group,then all the blastocysts suitable for freezing were vitrificated in the two groups. The blastocyst formation rate and vitrification frozen- thawed outcomes were compared between the two groups. Results： The high quality blastocyst formation rate in cleavage stage biopsy group（ 1. 64%） was statistically significantly lower than that in the blastocyst biopsy group（ 14. 75%）（ P = 0. 017）,but there was no statistically significant difference in the vitrification frozen- thawed recovery rate between the two groups. Conclusion： The poor quality cleavage embryos have a certain value for biopsy and blastocyst stage is more suitable for biopsy than cleavage stage.