蔷薇红景天低聚原花青素对D-半乳糖诱导小鼠过氧化损伤的拮抗作用

Study on antagonism of antioxidant effect of Rhodiola rosca oligomeric proanthocyanidns in deoxidization on mice induced by D-galactose

目的:探讨蔷薇红景天低聚原花青素(OPCRR)对D-半乳糖(D-gal)诱导小鼠过氧化损伤的拮抗作用。方法:采用D-半乳糖建立ICR小鼠过氧化模型中灌胃给予受试物连续49d,高、中、低剂量组分别添加蔷薇红景天原花青素320、160、80mg/kg·bw,考察血清和心、肝、脑组织中SOD、GSH、GSH-Px、MDA指标及肝、脑组织中的MAO指标。结果:与模型对照组相比,一定剂量的OPCRR可显著提高小鼠血清、心、肝、脑组织中SOD、GSH-Px活性,并显著降低MDA含量,且在高剂量组表现最突出;高剂量组血清、心、肝和脑组织中SOD活力分别增加了29.5%、28.1%、62.7%和90.8%,GSH-Px活性分别增加了67.8%、58.4%、32.1%和50.3%,MDA含量分别降低了19.2%、30.0%、33.3%和19.2%。此外,中、高剂量组极显著降低脑组织MAO活力(p<0.01),分别降低了22.3%和28.6%;高剂量组显著降低肝脏MAO活力(p<0.05),降低了36.5%。结论:OPCRR可一定程度地保护机体,拮抗D-gal的过氧化损伤,预防衰老。

Objective.To investigate the antioxidant effect of Rhodiola rosca oligomeric proantho cyanidns（OPCRR） in the aged mice induced by D-galactoseactose.Method..Via the process of establishing oxidative damage model by hypodermic injection of D-galactose, OPCRR of different doses （high dose, 320mg/kg. bw, middle dose.. 160 mg/kg-bw,low dose,,80 mg/kg, bw）were orally given to mice at the same time for consecutive 49d.The level of SOD, GSH-Px, M DA in mouse serum and organs, and senescent-related enzyme（MAO） in mouse brain and liver were investigated in peroxide damaged mice in the experiment of the antioxidant activity in vivo.Results.Compared with model control group,a dose of OPCRR could significantly increase SOD, GSH-Px activity of serum, heart, liver and brain,and significantly decrease MDA level, while highest close had superior effert~ high dose group had increased SOD level of serum, heart, liver and brain by 29..5 % ,29.5 % ,62.7% and 90.8 %, while GSH-Px activity had increased 67.8% ,58.4%, 32.1% and 50.3%, and the MDA content was decreased by 19.2%, 30.0%, 33.3% and 1.9.2%. In addition,the middle and high dose group significantly decreased MAO activity of brain （ p 〈 0.01 ） by 22.3 % and 28.6 % ; High dose group was significantly decreased MAO level of liver （ p 〈 0.05） by 36.5 %.Conclusion： OPCRR＇s effect on antagonizing D-galactoseactose-induced peroxidative damage was confirmed, and had a degree of ability of preventing oxidation and senescence.