黄连与人参配伍后生物碱的含量变化

Contents Change of Alkaloids in Coptidis Rhizoma by Different Proportions of Compatibility with Ginseng Radix et Rhizoma

目的:探讨黄连与人参不同配伍剂量对水煎液中盐酸小檗碱、盐酸药根碱含量的影响。方法:人参黄连分别按1∶0,2∶1,1.5∶1,0.5∶1比例配伍,煎煮制备水煎液,采用HPLC测定不同配伍剂量水煎液中黄连生物碱的含量。结果:人参-黄连不同配伍样品中盐酸小檗碱质量分数分别为36.68,39.41,44.33 mg·g-1,盐酸药根碱分别为8.607,8.349,10.790 mg·g-1,二者含量随人参用量的减少而增加。结论:黄连-人参配伍后不利于盐酸小檗碱和盐酸药根碱的溶出,按0.5∶1配伍时黄连中2种生物碱含量均最高。

Objective： To investigate contents change of jatrorrhizine hydrochloride and berberine hydrochloride in decoction by different proportions of compatibility of Coptidis Rhizoma and Ginseng Radix et Rhizoma. Method： Coptidis Rhizoma and Ginseng Radix et Rhizoma were made up a prescription by proportions of 2： 1, 1.5： 1, 0.5： 1, respectively; HPLC was adopted to determine contents of alkaloids in decoction. Result： The content of berberine hydrochloride were 36.68, 39.41, 44.33 mg·g^-1 in different proportions of compatibility of Coptidis Rhizoma and Ginseng Radix et Rhizoma, while the content of jatrorrhizine hydrochloride were 8. 607, 8. 349, 10. 790 mg·g^-1, these two components increased with decreasing dosage of Ginseng Radix et Rhizoma. Conclusion： Compatibility of Coptidis Rhizoma and Ginseng Radix et Rhizoma was not beneficial to dissolution of berberine hydrochloride and jatrorrhizine hydroehloride, compatibility of 0.5 ： 1 was most beneficial to dissolution of these two ingredients.