芍药与甘草配伍协同增效作用的物质基础研究

Study on Active Ingredients for Increased Efficacy of Compatibility of Penoy and Licorice

目的:探讨芍药与甘草配伍增效的化学物质基础。方法:芍药和甘草中主要活性成分采用HPLC检测;对方法学进行了专属性、线性范围、精密度、稳定性、重复性、加样回收率等考察。结果:芍药单味药单煎后芍药苷检测到的含量为2.19 mg·g-1;甘草单味药单煎后甘草酸检测到的含量为17.13 mg·g-1;芍药和甘草分别单煎再按1∶1混合,芍药苷检测到的含量为5.45 mg·g-1,甘草酸检测到的含量为17.69 mg·g-1;芍药和甘草按1∶1混合煎煮,芍药苷检测到的含量为2.58 mg·g-1,甘草酸检测到的含量为18.14 mg·g-1。结论:芍药与甘草配伍,两药单煎混合芍药苷和甘草酸的含量均显著提高,但前者增高更为明显;两药混合煎煮,芍药苷和甘草酸的含量均明显提高,但以后者更为显著;芍药与甘草配伍止痛的科学基础可能与两药合用后的芍药苷和甘草酸的含量增高有关。

Objective： To explore the chemical basis for co-application of Paeonia lactiflora and Glycyrrhizae uralensis. Method ： The contents of peoniflorin and glycyrrhizin were detected by using the method of high-performance liquid chromatography （HPLC） and the test methods in terms of specificity, linear range, precision, stability, repeatability and sample recovery were performed to determine the optimum chromatographic conditions. Result： The content of peoniflorin in P. lactiflora decoction was 2. 19 mg · g^-1; the content of glycyrrhizin in G. uralensis was 17.13 mg·g^-1 ; the contents of peoniflorin and glycyrrhizin were 5.45, 17.69 mg · g^-1 respectively when P. lactiflora and G. uralensis were mixed together after decocting the component herbs singly; the contentsof peoniflorin and glycyrrhizin were 5.45, 17.69 mg·g^-1 respectively when P. lactiflora and G. Uralensis were decocted together. Conclusion： The contents of peoniflorinand glycyrrhizinwere promoted significantly with the former more significant when the component herbs were decocted singly and the contents of peoniflorinand glycyrrhizin was elevated greatly with the latter more significant when the component herbs were decocted together; the increase in contents of peoniflorinand glycyrrhizin may compose one of important reasons behind the synegetic analgesic potency when P. lactiflora and G. uralensis are used together.