加味丹栀逍遥散对抑郁模型大鼠海马内盐皮质激素受体和糖皮质激素受体的影响

Effect of Jiawei Danzhi Xiaoyao Powder on Mineralocorticoid Receptor and Glucocorticoid Receptor in Hippocampus of Depression Rats

目的:观察加味丹栀逍遥散对抑郁模型大鼠海马内盐皮质激素受体(MR)和糖皮质激素受体(GR)含量的影响。方法:将50只Wistar雄性大鼠随机分为空白对照组、模型组、氟西汀组、加味丹栀逍遥散低剂量组、加味丹栀逍遥散高剂量组,每组10只;采用孤养结合慢性轻度不可预见性应激的复合模型,除空白组外,其余各组均接受不同应激因子刺激21 d,建立慢性应激抑郁大鼠模型,随后分别按氟西汀组2.5 mg·kg-1、加味丹栀逍遥散低、高剂量组2.04,4.08 g·kg-1,各组均按10 mL·kg-1ig给药,空白对照组和模型组ig 10 mL·kg-1生理盐水1次/d,每天应激前灌胃,从造模第22天开始,至动物处死当天,共21 d,实验周期共42 d;采用蛋白印迹法(Western-blot)测定大鼠海马内糖皮质激素受体(GR)和盐皮质激素受体(MR)的蛋白表达。结果:与空白组比较,模型组大鼠海马内GR的蛋白表达下调而MR的蛋白表达升高;经治疗后,各组大鼠海马内GR的蛋白表达较模型组显著上调(P<0.05),而各组大鼠海马内MR的蛋白表达较模型组显著下调(P<0.05)。结论:加味丹栀逍遥散可以通过调节海马内GR和MR的蛋白表达而起到抗抑郁的作用,经过药物干预后的模型大鼠海马内GR表达升高而MR的表达下调,其抗抑郁的作用机制可能与海马内GR和MR之间的比例关系失调有关。

Objective： To investigate the effects of Jiawei Danzhi Xiaoyao powder on the concentraion changes of mineralocorticoid receptor （MR） and glucocorticoid receptor （GR） in hippocampus ofdepression rats. Method： Fifty male Wistar rats were evenly randomized into control group, model group, fluoxertine group, modified Jiawei Danzhi Xiaoyao powder low dose, high dose, 10 in each group. Depression model of rat was produced by using chronic unpredictable mild stress stimulation combined with lonely raising for 21 days. From the twenty-second days of models, fluoxertine group 2.5 mg ·kg^-1, modified Jiawei Danzhi Xiaoyao powder low dose 2.04 g·kg^-1 , high dose 4.08 g·kg^-1 , each of the medication group perfused daily with the medicine which was needed 10 mL · kg^-1, and control group and model group perfused daily with normal saline of a volume the same as the medication group, before the stress of each day. Until all rats were killed, there are 21 days. Use the Western- blot to determine the expression of glucocorticoid receptor （GR） and mineralocorticoid receptor （MR） in the hippocampus of rats. Result： Compared with the control group, the down-relation of GR protein expression was found in hippocampus of model group, but MR protein expression increased. After treatment, the GR protein expression in hippocampus of rats significantly raised （P 〈 0.05） in the model group, and each group MR protein expression in hippocampus of rats from model group significantly lowered （P 〈 0.05）. Cortelusion： Jiawei Danzhi Xiaoyao powder can regulatet the GR and MR protein expression in hippocampus and antidepressant effect. Its mechanism of action of antidepressants may be related to the hippocampus in imbalance of the proportion of the relationship between GR and MR.