摘要
目的:观察益气解毒活络方对早期糖尿病肾病(diabetic nephropathy,DN)大鼠血流变和纤维蛋白原(Fib)及前列腺素E2(PGE2)的影响。方法:采用高脂高糖饲料喂养合并腹腔注射小剂量链尿佐菌素(streptozocin,STZ)方法建立糖尿病(DM)大鼠模型。以西药盐酸贝那普利作阳性对照,中药干预以益气解毒活络中药复方(12.78 g·kg-1)分别灌胃治疗8,10周。实验结束后,检测大鼠空腹血糖(FBG)、糖化血红蛋白(HbA1c)、血β2微球蛋白(β2-MG)及尿微量白蛋白(MALB);光镜观察肾组织形态结构变化(HE染色);血流变仪测定全血黏度(高切、中切、低切);凝血4项分析仪测定血浆Fib;酶联免疫吸附实验(Elisa法)检测血浆及肾组织PGE2的含量。结果:模型组大鼠FBG,HbA1c及血β2-MG、全血黏度、血浆Fib、尿MALB较正常组明显升高(P<0.05),PGE2含量升高(P<0.05);与模型组比较,各治疗组大鼠FBG,HbA1c及血β2-MG、全血黏度、血浆Fib、尿MALB显著降低(P<0.05),PGE2含量下降(P<0.05);模型组大鼠出现明显的肾脏形态学异常,各治疗组肾脏病变明显减轻。结论:DN的发病机制可能与血流变、Fib的异常及PGE2密切相关,益气解毒活络中药复方防治DN的发生发展可能是通过调节血流变、血浆Fib及纠正PGE2的异常实现的。
Objective： To investigate the effects of compound Chinese medicine Yiqi Jiedu Huoluo fang on hemorheology and fibrinogen （Fib） , prostaglandin E2 （PGE2） level in rats with diabetes nephropathy （DN）. Method： The rat model was established by feeding of HG and HF diet with peritoneal injection of streptozocin （STZ）. Interfere with benazepril （8 weeks） and herbs （12.78 g·kg^-1, 8, 10 weeks）. Fasting blood-glucose （FBG）, haemoglobin A1 c （ HbAlc ） and β2-microglobulin （β2-MG） and microalbuminuria （ MALB）, PGE2 （Elisa） were tested. The renal pathological changes were evaluated by HE staining. Observed the changes of hemorheology and Fib, PGE2. Result： The level of FBG, HbAlc,β2-MG, hemorheology and Fib, MALB, PGE2 in the model group were higher obvious （P 〈 0.05） , the other group were lowed. The renal pathological changes were obviously in the model group, and the therapy group were all alleviated. Conclusion： The pathological changes of kidney tissue have a realationship with hemorheology and Fib, PGE2 , herbs of Yiqi Jiedu Huoluo fang in percent and cure to diabetic mellitus （DN） may by regulating the abnormal of hemorheology and Fib and PGE2.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第11期165-169,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81173153)
