补肾化瘀中药干预对光老化大鼠模型表皮及真皮结构的影响

The influence of replenishing kidney dissolving blood stasis therapy on epidermis and dermis structure of photoaging skin rat models

目的探讨补肾化瘀中药干预对光老化大鼠模型表皮及真皮结构的影响。方法利用8-甲氧补骨脂素/长波紫外线UVA（320-400 nm）照射SD大鼠,建立光老化大鼠模型,并对该动物模型给予补肾化瘀中药干预,连续15 d。40只SD大鼠分为空白对照组（实验过程中不经紫外线照射和任何干预）10只、补肾化瘀中药干预组（紫外线照射造模后,给予补肾化瘀中药干预）15只、模型对照组（紫外线照射造模后,给予生理盐水灌胃）15只。采用皮肤组织病理学对3组大鼠的表皮及真皮结构改变进行观察和分析。结果成功建立光老化大鼠模型,补肾化瘀中药干预组大鼠损伤区皮肤外观及弹性趋向正常,组织形态学上光老化损伤表现基本消失,真皮层出现新生胶原纤维,排列紧密,弹力纤维分布均匀。结论补肾化瘀中药具有抗光老化作用,能够促进光老化大鼠皮肤损害的修复,为补肾化瘀中药抗光老化研究提供了客观的组织形态学依据。

Objective To study on the influence of replenishing kidney dissolving blood stasistherapy on epidermis and dermis structure of photoaging skin rat models. Methods Using 8- methoxypsoralan / UVA ray（ 320 - 400 nm） irradiation was applied on SD rats for establishment of skin photoaging rat model. The intervention of replenishing kidney dissolving blood stasis therapy was given for 15 days in a row. Histopathological observation was performed for analysis of the changes of epidermis and the dermis structure of rats in control group,photoaging group and intervention group. Results The photoaging rat model was established successfully. The tended normal appearance and elasticity of damaged areas,almost disappeared glazing aging damage performance,new densely arranged dermis collagen fiber,and evenly distributed elastic fiber were observed in therapy group. Conclusion Replenishing kidney dissolving blood stasis therapy provides anti- photoaging effect in photoaging rats,and promotes the repairmen of impaired skins.