白背飞虱羧酸酯酶与乙酰胆碱酯酶的体躯与亚细胞分布特征

Distribution patterns of carboxylesterase and acerylcholinesterase in the white backed planthopper, Sogatella furcifera (Hemiptera: Delphacidae).

白背飞虱 Sogatella furcifera( Horváth)羧酸酯酶 ( Ca E)和乙酰胆碱酯酶 ( Ach E)的体躯及亚细胞分布特征研究结果表明 ,若虫头、胸、腹部中 Ca E活性分别占 50 .8% ,2 6.0 %和 2 3.2 % ,雌成虫各占33.0 % ,2 3.5%和 4 3.5% ,雄成虫各占 2 6.5% ,2 3.2 %和 50 .3%。若虫、雌雄成虫的 Ach E活性均主要分布于头部 ,各占总活性的 87.7% ,71.1%和 74 .7%。差速离心结果表明 ,该飞虱 Ca E主要位于胞质溶胶层中 ,其活性占 57.0 %～ 66.9% ;Ach E活性在胞质溶胶层中仅占 5.8%～ 17.0 % ,而 62 .8%～ 76.9%的活性则分布于线粒体层、细胞核和细胞碎片层中。

To determine both body and subcellular distributions of carboxylesterase (CaE) and acetylcholinesterase (AchE) in the white backed planthopper, Sogatella furcifera (Horváth), the results showed that the percentages of CaE specific activity in the head, thorax and abdomen were 50 8%, 26 0% and 23 2% of total activity in the 5th instar nymph, while 33 0%, 23 5% and 43 5% in the female adults, as well as 26 5%, 23 2% and 50 3% in the male, respectively The AchE in the head had the highest specific activity, which ranked 87 7% in the nymph, 71 1% in the female and 74 7% in the male The results from the differential centrifugation indicated that the major portion of CaE with 57 0% 66 9% of total activity located in the cytosol fraction, whereas only 5 8% 17 0% of the AchE distributed in the cytosol fraction, and about 62 8% 76 9% in the part of mitochondrial fraction and nuclei and cell debris Finally the forms of CaE and AchE existed in the cell of this planthopper were discussed