桃核承气汤对脓毒症大鼠肝脏组织的保护作用及机制

Hepatic Protective Effect and Mechanism of Taohe Chengqi Decoction in Sepsis Rats

目的:观察桃核承气汤对脓毒症大鼠肝脏组织的的保护作用及机制。方法:将大鼠100只随机分为正常对照组(NC)、模型6 h组(MC6h)、模型12 h组(MC12h)和桃核承气汤6 h组(Th6h)、桃核承气汤12 h组(Th12h),每组20只。NC不作任何处理,MC每只大鼠每天按1 mL/100 g体重灌胃给予生理盐水,Th每只大鼠每天按1 mL/100 g体重予桃核承气汤药液灌胃,7天后造模。大鼠造模成功后6 h、12 h分别采集血清、肝组织标本,行一般生化检查及病理染色,电镜观察肝组织改变情况。结果:①与NC比较,MC6h和MC12h谷丙转氨酶(ALT)和谷草转氨酶(AST)水平明显升高,差异均有非常显著性意义(P<0.01);与同时间点MC比较,Th6h、Th12 h实验大鼠的血清ALT和AST水平均降低,差异均有显著性意义(P<0.05)。②与NC比较,MC6h和MC12h肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)和内毒素(LPS)的水平明显升高,差异均有非常显著性意义(P<0.01);与同时间点MC比较,Th6h、Th12 h TNF-α、IL-6和LPS的水平均降低,差异有显著性或非常显著性意义(P<0.01,P<0.05)。③Th12h大鼠肝脏组织的受损程度明显减轻,与MC12h比较,差异有显著性意义(P<0.05)。光镜和电镜观察MC12h大部分肝组织细胞严重水肿,肝细胞点灶性坏死,汇管区淋巴细胞浸润。结论:桃核承气汤可减轻脓毒症大鼠肝组织炎性损伤,其机制可能与减少TNF-α、IL-6、LPS等炎症因子和体内LPS的水平有关。

objective： To observe the protective effect and mechanism of Taohe Chengqi decoction on liver tissue in rats with sepsis. Methods： A total of 100 male clean Kunming SD rats were randomly divided into five groups, the normal control group（NC）, the model 6h group（MC6h）, the model 12h group（MC12h）, Taohe Chengqi decoction 6h group（Th6h） and Taohe Chengqi decoction 12h group（Th12h）, 20 in each group. The normal control group had no treatment. Each rat of model group was given saline lmL/100g（body weight） per day through gastrogavage. The rat of Taohe Chengqi decoction group was given Taohe Chengqi decoction 1mL/100g（body weight） per day through gastrogavage. The modeling lasted 7 days. Serum and liver tissue specimens were collected respectively at 6 h and 12 h after the modeling, and were observed by biochemical examination, pathological dyeing and electronic microscopy. Results： The levels of serum aminotransferase （ALT） and aspartate aminotransferase（AST） in MC6h group and MC12h group were increased（P 〈 0.01 compared with those in the NC group at the same time point）. The levels of serum ALT and AST in Th6h group and Th12h group were lower than those in the MC group at the same time point（P 〈 0.05）. The levels of serum tumor necrosis factor-α （TNF-α）, interleukin-6（IL-6） and lipopolysaccharide（LPS） in MC6h and MC12h groups were higher than those in the NC group at the same time point（P 〈 0.01）. Rats in Th6h and Th12h groups had lower serum TNF-α, IL-6 and LPS levels than MC groups at the same time point（P 〈 0.05 or P 〈 0.01）. The damage of liver tissue in Th12h group was relieved obviously（P 〈 0.05 compared with that in MC12h group at the same time point）. MC12h group had severe edema of liver tissue and spotty necrosis of liver cells, infiltration of lymphocytes into portal area. Conclusion.. Taohe Chengqi decoction can reduce the liver inflammatory damage of rats with sepsis , and the mechanism may be related with decreasing inflammatory factors of TNF- α, IL-6 and LPS level.