水稻穗长上位性效应和QE互作效应的QTL遗传研究(英文)

Study on Epistatic Effects and QTL × Environment Interaction Effects of QTLs for Panicle Length in Rice ( Oryza sativa L )

利用基于混合模型的 QTL定位方法研究了由籼稻品种 IR64和粳稻品种 Azucena杂交衍生的DH群体在四个环境中穗长的 QTL上位性效应和环境互作效应。结果表明上位性可能是数量性状的重要遗传基础 ,并揭示了上位性的几个重要特点。在本研究中 ,所有的 QTL中只有两个没有参与上位性效应的形成 ,在参与上位性效应的 QTL中 ,64.7%的 QTL还具有本身的加性效应。因此传统方法对 QTL加性效应的估算会由于上位性的影响而有偏。其它 35.3%的 QTL没有本身的加性效应 ,却参与了上位性互作 ,这些位点可能通过诱发和修饰其它位点而起作用。上位性的特点还包括 ,经常发现一个 QTL与多个 QTL发生互作 ;大效应的 QTL也参与上位性互作 ;上位性互作易受环境影响。QTL与环境的互作效应比 QTL的主效应更多次地被检测到 。

QTLs with epistatic effects and environmental interaction effects for panicle length of rice were studied by mixed model based QTL mapping with a doubled haploid population from IR64/Azucena in four environments The results demonstrated the importance of epistasis as a genetic basis of the quantitative traits and also revealed several important features of this phenomenon In the results, all QTLs except two were involved in epistasis, and 64 7 per cent of the QTLs involved in epistasis were found with significant additive effects This might mean that the usual estimates of the QTL additive effects could be confounded by epistatic interactions and result in biased estimation The other 35 3 per cent QTLs did not have any significant additive effects of their own but were also involved in epistatic interactions Such loci might play the role of modifying agents that tend to activate other loci or modify the action of other loci The other features of epistasis include that,it was fairly common for the same locus to get involved in interactions with more than one other locus; and epistatic interactions were sensitive to environmental affections QE effects were detected more often than QTL main effects, might indicate that gene expression for quantitative trait could be greatly affected by environments