绍鸭腺胃蛙皮素细胞生长发育的免疫组织化学观测

Immunohistochemical study of the development of P cells in the proventriculus of shao ducks

采用免疫组织化学 ABC方法 ,显示绍兴麻鸭胚后期腺胃蛙皮素免疫反应细胞 ( P细胞 )的形态及其分布 ,分别计数每个周龄阶段的腺胃腺叶和表面上皮中蛙皮素免疫反应细胞的数量 ,并进行了统计学分析。结果表明 :P细胞形态多样 ,以卵圆形、多面体形为主 ,并且有胞质突起。P细胞多分散分布于腺叶的中间与内侧区 ,个别分布在表面上皮中。腺叶 P细胞的生长峰期为 0～ 6周龄 ,以后下降 ,表面上皮P细胞生长峰期为 0～ 10周龄 ,以后迅速下降。各周龄腺叶 P细胞数明显多于表面上皮 P细胞数。

Immunohistochemical method (ABC method) was employed to demonstrate the postnatal development of bombesin cells (P cells) in proventriculus of Shao duck by observing its character and counting its number in every stage. The results presented that the P cells were mainly oval and polygonal, and some with short cytoplasmic processes. Most of P cells disperse in the central area of gastric glands and only a few located in the epithelial surface of lumen. The P cells' growth peak of gastric gland appeared during 0 6 weeks, and the epithelial did during 0 10 weeks. Following the growth peak, both gastric P cells and epithelial decreased quickly. The results also showed that the gastric P cells were remarkably more than the epithelial. This paper presented the postnatal development of the gastric P cells in Shao ducks. It also provided basic data for studying the dynamic change of the gastroenteropancreatic endocrine system.