摘要
目的通过对大鼠皮肤溃疡愈合过程中肉芽组织TGF-Β1和TIMP-1含量的动态观察,探讨复方落地生根膏促进大鼠溃疡愈合的机制。方法制备大鼠创伤性溃疡模型120只,随机分为对照组、实验对照组和实验组,每组各40只。三组分别用生理盐水纱块、生长因子纱块和复方落地生肌膏外敷伤口,实验对象每日肌注青霉素8000u。每组40只SD大鼠随机分4次、每次10只于实验的1、7、14及皮肤愈合时取材。采用RT-PCR法检测TGF-Β1和TIMP-1含量。结果实验组和实验对照组能显著缩短创面愈合时间(P<0.05),改善瘢痕质量;TGF-Β1/TIMP-1在溃疡愈合过程中的表达具有时间相关性,TGF-Β1表达趋势呈抛物线样改变,高峰出现在创面愈合中期,而TIMP-1表达呈直线上升,高峰出现在愈合晚期。结论复方落地生根膏可通过调节组织愈合过程中TGF-Β1和TIMP-1的表达而促进创面愈合并改善瘢痕质量,与常用的生长因子相比,具有简便廉验的特点,值得临床推广。
Objective To explore the mechanism of ulcer healing in rats dy observation the expression of TGF -Β1 and TIMP-1 in granulation tissue.Methods The 120 rat models were randomly divided into control group , the experimental control group and experimental group , n=40.The three groups were respectively treated by saline , growth factor and Luodishengen and each rat was injected penicillin 8000u.On the 1st、7th、14thand healing day,the expression of TGF-Β1 and TIMP-1 were observed.Results Expression of TGF-Β1 /TIMP-1 was time-dependent in ulcer healing process , Expression of TGF-Β1 was parabolic trend, peaked in the interim,but TIMP-1 expression was straight up, peaked in the healing time .Conclusion By regulation the expression of TGF-Β1 and TIMP-1, Luodishenggen can promote wound healing and improve scar quality .
出处
《光明中医》
2014年第5期947-950,共4页
GUANGMING JOURNAL OF CHINESE MEDICINE
