制苗用鸡大肠杆菌培养基及培养方法

Medium and Cultural Method for Preparing Avian E.coli Vaccine

采用通气搅拌培养方法对鸡大肠杆菌用 MM培养基和改良 MM培养基进行了对比试验 ,并用改良MM培养基对鸡大肠杆菌进行了静止培养、通气搅拌培养和连续通气搅拌培养的对比试验。结果显示 ,改良MM培养基培养的鸡大肠杆菌菌数和光密度 ( D)值明显高于 MM培养基 ;连续通气搅拌培养和通气搅拌培养的方法明显优于静止培养方法。

Medium and cultural method for preparing avian E.coli vaccine were studied by using MM medium and improved MM medium.The contrast tests were performed by using static,airing stir and continuing stir culture.The results showed that avian E.coli count and D 600 value in improved MM medium were obviously higher than in MM mediam.and that both the airing stir and continuing stir culture were significantly better than the static culture.