牛胚胎移植技术在中国内蒙古的研究和应用

Research and Application of Cattle Embryo Transfer Technique in Inner Mongolia of China

1 991～ 1 996年 ,对 6个品种 1 38头供体母牛超排处理 1 99次 ,共获得 A、B级胚胎 1 1 4 8枚 ,平均每次每头获可用胚 5.77枚 ;共移植受体 1 2 99头 (包括鲜胚和冻胚 ) ,妊娠产犊 593头 ,总移植妊娠率为 4 5.7%。对牛的超数排卵技术进行了深入研究 ,对不同超排方法的超排效果、不同品种肉牛的超排反应及重复超排的效果进行了分析 ,获得了适于重复应用、效果稳定的超排方案 ,即用 FSH-PG法 ,FSH总剂量在 4～ 6mg,5d 1 0次递减注射。同时 ,对受体牛饲养管理条件与受体诱导同期发情效果和移植妊娠率之间的关系进行了探讨 ,对经产牛和育成牛受体移植妊娠效果进行了比较 ,并对移植人员技术熟练程度对妊娠率的影响进行了分析 ,从而为大规模实施牛

From 1991 to 1996,138 donor cows that cover 6 breeds were superovulated 199 times. 1 148 A and B grade embryos were obtained.The average number of A and B grade embryos was 5.77 /head/time. 1 299 recipients(including fresh embryos and frozen embryos).593 ET offspring were born.The total pregnant rate of transferring was 45.7 percent.We analysed the effect of different superovulation method,the reaction of different breeds of donors to superovulation and the effect of repeat superovulation and obtained the effective and stable superovulation method that fitted repeat superovulation of donors.In the meanwhile,we studied initially the relation between the nutrition level of recipients and the effect of induced sychronous estrus of recipients and the relation between the nutritious level of recipients and the pregnant rate of transferring and compared the pregnant rate of cow recipients with that of heifer recipients,and analysed the influence of technician skill on the pregnant rate of transferring.A selection criterion of recipients was provided for conducting cattle ET technology on a large scale.