柿品种‘禅寺丸’未减数花粉分离体系的建立

Study on the Establishment of Separation System for Unreduced Pollens in ‘Zenjimaru' Persimmon Variety

对柿品种'禅寺丸'未减数花粉（2n花粉）的鉴定、筛选及其生活力恢复进行了研究，结果表明：（1）经0．9 mol／L甘露醇CPW溶液吸胀2h后，正常花粉直径为50 μm左右，而未减数花粉为65 μm左右，未减数花粉直径是正常花粉的1．3倍。（2）未减数花粉比率约为3．8％，通过61μm的不锈钢细胞筛筛选，纯度可达84％。（3）禅寺丸花粉经吸胀处理后，花粉发芽率有所下降，利用20％的蔗糖溶液洗涤可基本恢复其生活力。

Studies were carried out on the identification，screening and recovery of viability of unreduced pollen （2n pollen） in Japanese 'Zenjimaru' persimmon variety  Diospyros kaki L． f ．The results showed that 1 The diameter of normal pollen and unreduced pollen was 50 μm and 65 μm，respectivelyafter imbibed about 2 hours in CPW solution added 0．9 mol／L mannitol． Unreduced pollen was 1．3 times to normal one in diameter．2Unreduced pollen percentage was 3．8％ naturally and could be increased to 84％ after screened by stainless steel mesh．3The viability of pollen declined after imbibed  but could recover partly by 20％ sucrose solution．