尿道及其他显色培养基快速分离鉴定常见泌尿道感染致病菌的临床应用

Urethra and other chromogenic medium fast isolation and identification of common urinary tract infection pathogens in clinical application

目的:总结尿道及其他显色培养基快速分离鉴定常见泌尿道致病菌以避免混合菌引起错误的鉴定及药敏结果。方法:按照《全国临床检验操作规程》第3版尿培养的方法;标本直接接种在血平板及尿道显色培养基等平板上进行培养鉴定,用法国生物梅里埃公司ATBExpression自动细菌鉴定仪进行鉴定和/或验证。结果:主要泌尿道感染菌49.1%为大肠埃希菌,8.9%为肺炎克雷伯菌,10.8%为变形杆菌。结论:经验证明总符合率为96%,尿道显色培养基能快速、方便分离鉴定常见泌尿道致病菌,节约试剂成本,适合各基层医院推广应用。

Objective Urethra and other chromogenic medium fast identification of common urinary tract pathogens isolated in order to avoid errors caused by mixed bacteria identification and susceptibility results. Methods Accordance with the ＂ national clinical laboratory Procedures＂ 3rd edition urine culture methods used; specimens inoculated directly on blood agar and urethra chromogenic media for culture and identification plates,etc. with the French bioMérieux atbexpression automatic identification system for identification of bacteria and / or verification. Results Major urinary tract infection escherichia coli bacteria 49. 1%,klebsiella pneumoniae 8. 9%,proteus 10. 8%. Conclusion Proven compliance rate of 90% to 100%. Urethral chromogenic medium can quickly and easily isolated and identified common urinary tract pathogens,saving the cost of reagents,suitable for all basic hospital application.