博落回花药离体培养及植株再生研究

In Vitro Culture of Anther and Plant Regeneration for Macleaya cordata

对8份不同产地的博落回花药进行了组织培养，通过对花药来源、4℃冷激时间、35℃热激时间、取蕾时期等因素的研究，建立了有效的博落回花药离体培养胚状体发生和植株再生体系。结果表明：有6个产地的博落回花药试材可以获得胚状体和再生植株；不同产地间出胚率差异显著，其中来自湖南高坊的材料出胚率最高，为11.8％；在4℃下冷激24~96 h有利于诱导出胚状体，冷激48 h的花药最容易产生胚状体；35℃热激处理对提高胚状体诱导率的作用不明显；花药培养的最佳取蕾时期为盛花期。

A tissue culture was conducted to eight samples ofMacleaya cordata from different production areas, and the effective in vitro culture of anther and plant regeneration system forMacleaya cordata were established through studied the resources of anther, the cold-stress time （4℃）, the heat-stress time （35℃）, and the bud harvest time. The results showed that the anther samples ofMacleaya cordata from six production areas can obtain embryoid and regenerated plant; there was significant difference in embryo rate among different production areas, among which the sample from Gaofang of Hunan province had the highest embryo rate by 11.8%; cold-stress of anther at 4℃ for 24~96 h was beneficial for inducing embryoid, and the anther under cold-stress for 48 h was the most easy one to produce embryoid; the heat-stress treatment （35℃） had not signiifcant effect on improving inducing rate of embryoid; the optimal bud harvest time for anther culture was the full-bloom stage.