乙型肝炎病毒及黄曲霉毒素暴露的肝细胞癌中β-catenin、PTEN的mRNA表达

The mRNA expression of β-catenin and PTENin Hepatocellular carcinoma exposed to hepatitis B virus and aflatoxin B1

目的研究β-catenin、PTEN基因在乙型肝炎病毒(HBV)及黄曲霉毒素B1(AFB1)暴露下肝细胞癌(HCC)中mRNA表达,探讨在双暴露情况下β-catenin、PTEN与HCC发生、发展的关系。方法根据HBV与AFB1的暴露情况,将108例肝细胞癌手术切除研究对象分为4组,A组:HBV(+)/AFB1(+)48例;B组:HBV(+)/AFB1(-)27例;C组:HBV(-)/AFB1(+)19例;D组:HBV(-)/AFB1(-)14例。同时收集正常肝组织者20例,分别来自肝外伤、肝血管瘤、肝移植供体等手术切除标本作为正常对照组。采用逆转录-PCR(RT-PCR)法检测β-catenin基因与PTEN基因的mRNA表达情况,分别对各组的表达情况进行比较。结果 RT-PCR显示,β-catenin基因mRNA的半定量平均灰度值A组(1.13±0.14)、C组(1.16±0.18)分别与D组(1.01±0.13)相比,差异有统计学意义(P<0.05),此外,A、B(1.06±0.18)、C、D组与正常对照组比较,差异均有统计学意义(P<0.001)。PTEN基因mRNA的半定量平均灰度值A组(0.54±0.13)、B组(0.59±0.16)分别与C组(0.97±0.16)及D组(0.92±0.13)相比,差异有统计学意义(P<0.05)。此外,A、B、D组与正常对照组相比,差异有统计学意义(P<0.05)。结论β-catenin基因的高表达率可能与AFB1高暴露有关,PTEN基因的失活与HBV高感染率有关。

Objective To investigate the mRNA expression of β‐catenin and PTEN in hepatocellular carcinoma exposed to hepa‐titis B virus（HBV） and aflatoxin B1（AFB1） .Methods 108 HCCs came from different districts of Guangxi province were labeled as four categories based on their biomarkers of HBV and AFB1 exposure .Group A ：HBV（＋ ）/AFB1（＋ ） ,48 cases ,group B ：HBV （＋ ）/AFB1（-） ,27 cases ,group C ：HBV（-）/AFB1（＋ ） ,19 cases ,group D ：HBV（-）/AFB1（-） ,14 cases .And normal hepatic tissue from 20 cases of hepatic hemangioma ,liver resection and liver transplant donor were chosen as normal control group .And the mRNA expression of β‐catenin and PTEN were detected by RT‐PCR .Results The mean expression level of β‐catenin gene mRNA in group A ,B ,C ,D and control group were（1 .13 ± 0 .14） ,（1 .06 ± 0 .12） ,（1 .16 ± 0 .18） ,（1 .01 ± 0 .13） and（0 .085 ± 0 .13） respec‐tively .There were significant differences between group A and C ,A and D .And there were significant differences between these four groups and control group（all P〈0 .05） .The mean expression level of PTEN gene mRNA in four subgroup A ,B ,C ,D and con‐trol group were（0 .54 ± 0 .13） ,（0 .59 ± 0 .16） ,（0 .97 ± 0 .16） ,（0 .92 ± 0 .13） and（1 .10 ± 0 .16） respectively .There were significant differences between group A and D ,C and D .And there were significant differences between group A and C （P=0 .002） ,A and D（P=0 .032） ,B and C（P〈0 .001） and B and D（P=0 .011） .And there were significant differences between subgroup A ,B and D and control group（all P〈0 .05） .Conclusion The over expression β‐catenin of HCC cases may be associated with the exposure to AFB1 while the loss of gene PTEN may relate to the exposure to HBV .