一株分离自冻墨鱼的溶藻弧菌的鉴定及其低温下细胞脂肪酸的变化

Identification and Changes in Membrane Fatty Acid Composition at Low Temperature in Vibrio alginolyticus from Frozen Cuttlefish Sepiella maindroni

对冻墨鱼中分离的菌株C6-1进行种属鉴定,并分析其低温状态下脂肪酸组成变化。通过VITEK 2Compact全自动微生物鉴定仪分析菌株的生理生化特征;16SrRNA基因序列同源性分析确定该菌株的系统发育学地位;通过气相色谱和质谱联用法分析菌株的全细胞脂肪酸。菌株C6-1为革兰氏阴性菌,对弧菌抑制剂O/129敏感;生理生化特征与溶藻弧菌的相似性为98%,16SrRNA序列与溶藻弧菌(GenBank No.JF836167)的相似性为99%;菌株的主要脂肪酸组成为C12:0,C14:0,C16:1(n-7),2甲基-C17:0,C18:0和C18:1(n-9);低温培养状态下,短链饱和脂肪酸(碳链长度小于16)相对含量由14.8%增至44.1%。

Bacterial strain C6‐1 isolated from cuttlefish Sepiella maindroni was characterized and changes in membrane fatty acid composition of the bacterial strain C 6‐1 in response to low temperature were also studied . The physiological and biochemical characteristics were analyzed using VITEK 2 compact automated microbiology system . The 16S rRNA gene was sequenced and subjected to phylogenetic analysis ,and the fatty acids were detected by gas chromatography‐mass spectrometry （GC‐MS） .It was found that strain C6‐1 was Gram‐negative and susceptible to the vibriostatic agent O/129 ,that exhibited the maximal level of 98% probability to Vibrio alginolyticus based on the conventional physiological test . The sequence analysis of 16S rRNA gene of C6‐1 isolation showed that C6‐1 was very close to V . alginolyticus （GenBank No .JF836167） with similarity of 99% .The major fatty acids were shown to be C12 ：0 ,C14：0 ,C16 ：0 ,C16：1（n‐7） ,iso‐C17 ：0 ,C18 ：0 and C18 ：1 （n‐9） .The major change in fatty acid composition in response to low temperature was characterized by increase in fatty acids with chain lengths less than 16 carbons from 14 .8% to 44 .1% .