高效液相色谱法测定人尿微量白蛋白的临床应用研究

Clinical application of high performance liquid chromatography to detect minute quality albumin in humanurine

目的探讨高效液相色谱法测定人尿微量白蛋白的临床应用价值。方法采集我科室自2012年10月至2013年10月进行尿微量白蛋白检测180例,分别进行高效液相色谱法和免疫比浊法进行临床对比分析,观察其检验结果。结果对尿液微量白蛋白行高效液相色谱法检测,出峰时间为1.7 min,而且非常稳定。检测低限是2mg/L,有较满意的精密度和准确度。180例患者中,高效液相色谱法能检出96例微量白蛋白尿(检出率53.3%),免疫散射比浊法检出48例微量白蛋白尿(检出率26.7%),高效液相色谱法比免疫散射比浊法检出率更高。高效液相色谱法各份标本的结果均高于免疫散射比浊法。结论高效液相色谱法检测快速,流动相配制简单,灵敏度高,线性范围宽,结果准确,能检出更多的尿微量白蛋白,可以为糖尿病肾病的早期检测提供客观的科学依据,有较大的临床应用价值。

Objective To detect minute quality albumin in human urine by high performanee liquid chromatography （HPLC）. Methods One hundrend and eighty cases of minute quality albumin detection in human urine had been collected from October 2012 to October 2013. HPLC and immunoturbidimetry were performed respectively on these cases to contrast the testing results. Results The peak time of minute quality albumin in human urine by HPLC was 1.7 minute with stable feature. The detection lower limit was 2 mg/L with favorable precision and correctness. Ninty-six minute quality albumin eases were detected by HPLC in the 180 cases （detection rate=53.3% ）, whereas only 48 were detected by immunoturbidimetry （detection rate=26.7% ）. The detection rate results by HPLC was clearly higher than those by immunoturbidimetry. Conclusion HPLC has the advantages of rapid detection, simple mobile phase preparation, high sensitivity, wide linear range and reliable results. It can be applied to detect diabetes and kidney disorders at early stage, which provides objective basis for their elinieal diagnosis.