子宫肌瘤患者外周血胰岛素样生长因子水平的变化及其与子宫肌瘤组织中雌激素受体和孕激素受体的关系研究

Changes of IGF in Peripheral Blood of Patients with Uterine Leiomyoma and Its Relation with ER and PR in Uterine Leiomyoma Tissues

目的探讨子宫肌瘤患者外周血胰岛素样生长因子(IGF)-Ⅰ和IGF-Ⅱ水平的变化及其与子宫肌瘤组织雌激素受体(ER)、孕激素受体(PR)的关系。方法选择2013年1—9月在汕头大学医学院第一附属医院妇产科住院手术治疗临床诊断为子宫肌瘤的患者56例为研究组,另选择同期在本院进行健康体检的45例健康女性为对照组。采用酶联免疫吸附试验法测定研究组及对照组外周血IGF-Ⅰ、IGF-Ⅱ水平,采用免疫组化方法测定研究组子宫肌瘤组织ER和PR的表达情况,分析子宫肌瘤患者外周血IGF-Ⅰ、IGF-Ⅱ水平与子宫肌瘤组织中ER、PR表达的相关性。结果研究组外周血IGF-Ⅰ和IGF-Ⅱ水平均高于对照组(P<0.05)。子宫肌瘤患者ER(+)组外周血IGF-Ⅰ和IGF-Ⅱ水平均高于ER(-)组(P<0.05);子宫肌瘤患者PR(+)组外周血IGF-Ⅰ和IGF-Ⅱ水平均高于PR(-)组(P<0.05)。相关性分析结果显示,子宫肌瘤患者外周血IGF-Ⅰ和IGF-Ⅱ水平与子宫肌瘤组织中ER、PR表达评分均呈正相关(P<0.05)。结论子宫肌瘤的发生和发展可能与患者外周血IGF-Ⅰ和IGF-Ⅱ水平升高有关,并且与子宫肌瘤ER、PR的表达相关。

Objective To study the changes of insulin- like growth factor（IGF）- Ⅰ and IGF- Ⅱ in peripheral blood of patients with uterine leiomyoma and its relation with estrogen receptor（ER） and progestrone receptor（PR） in uterine leiomyoma tissues.Methods 56 patients diagnosed as leiomyoma in the department ofgynecology of the First Affiliated Hospital of Shantou University Medical College from January to September in 2013 were selected as the study group,while 45 healthy women having physical examination over the same period were selected as control group.The levels of IGF- Ⅰ and IGF- Ⅱ in peripheral blood in patients with uterine leiomyoma were determined by enzyme linked immunosorbent assay,and the expression of ER and PR in uterine leiomyoma tissues were determined by immunohistochemistry methods.The relation between the levels of IGF- Ⅰ and IGF- Ⅱ and the expression of ER and PR in uterine leiomyoma tissues was analyzed.Results The levels of IGF- Ⅰ and IGF- Ⅱ in the study group were significantly higher than the control group（P〈0.05）.The levels of IGF- Ⅰ and IGF- Ⅱ in ER（＋） patients were significantly higher than the ER（-） patients（P〈0.05）.The levels of IGF- Ⅰ and IGF- Ⅱ in PR（＋） patients were significantly higher than the PR（-） patients（P〈0.05）.Correlation analysis showed that the levels of IGF- Ⅰ and IGF- Ⅱ in patients with uterine leiomyoma were positively correlated with ER and PR（P〈0.05）.Conclusion The occurrence and development of uterine leiomyoma are probably related with the levels of IGF- Ⅰ and IGF- Ⅱ in the peripheral blood of patients,and are also related with the expression of ER and PR.