化学计量学及双标线性校正法应用于马钱子指纹图谱的研究

Fingerprint Analysis of Semen Strychni by Chemometrics and Linear Calibration Using Two Reference Substances

目的建立马钱子药材指纹图谱,并采用化学计量学方法和双标线性校正法进行研究。方法采用HPLC建立马钱子指纹图谱,使用相似度分析、二维聚类分析和主成分分析进行质量评价,并使用双标线性校正法进行色谱峰定性。结果 10批次马钱子药材选定14个色谱峰作为指纹图谱的共有峰,进一步选择马钱苷酸、绿原酸、士的宁和马钱子碱4个色谱峰作为指纹图谱的特征峰。经化学计量学评价,可对其产地进行初步分类。双标线性校正法预测保留时间的精度优于相对保留时间法。结论本实验建立的指纹图谱有较强的针对性,可用于马钱子的质量评价。与相对保留时间法相比,双标线性校正法预测保留时间结果的准确度更高,色谱柱的适用范围更广。

OBJECTIVE To establish the HPLC fingerprint of Semen Strychni by chemometrics and the method of linear calibration using two reference substances（ LCTRS）. METHODS The fingerprint of Semen Strychni was established by HPLC,using similarity analysis,2-dimensional clustering analysis and principal component analysis for quality evaluation. LCTRS was used for identification of chromatographic peak. RESULTS Fourteen chromatographic peaks were selected as common peaks in the fingerprints of 10 batches of Semen Strychni,and the chromatographic peaks of loganic acid,chlorogenic acid,strychnine and brucine were selected as characteristic peaks. By means of chemometrics evaluation,10 batches of Semen Strychni were classified by their origins. The precision of predicting retention time by LCTRS was superior to the relative retention time method. CONCLUSION LCTRS is more accurate for predicting retention time and suitable for more kinds of chromatographic columns than relative retention time method. In addition,the established fingerprint is specific,and can be used for the quality evaluation of Semen Strychni.