抗癌灵活性部位对不同肿瘤细胞的抑制作用

Anti-tumor Effect of Kang Ai Ling's Active Site on Different Cancer Cells

目的研究抗癌灵正丁醇提取部位(以下简称KAL)对多种肿瘤细胞的抑制率以及时效和量效关系。方法采用MTT法考察KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60的抑制率以及IC50;通过观察KAL对肿瘤细胞SGC-7901和PC-3生长的影响,考察其时效和量效关系。结果 KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60的IC50分别为:(21.05±4.31),(13.43±4.78),(15.17±3.82),(26.39±5.76),(25.78±4.40)μg·mL-1,且在抑制肿瘤细胞过程中呈现明显的时效关系和量效关系。结论 KAL对肿瘤细胞B-16、SGC-7901、Colo320、PC-3、HL60具有显著抑制作用。

OBJECTIVE To observe the growth inhibitory effect of tumor cells by Kang Ai Ling＇s 1-butanol exrtract（KAL） METHODS The anti-proliferative activities of 1-butanol extract of KAL against B-16, SGC-7901, Colo320, PC-3 and HL60 cells were evaluated by MTT colorimetric assay in vitro, then the result showed by inhibitory rate and IC50. Investigation on KAL＇s dose-effect dependent manner and time-effect dependent manner through growth inhibitory curve on SGC-7901 and PC-3. RESULTS The IC50 of KAL on B-16, SGC-7901, Colo320, PC-3 and HL60 were （21.05±4.31）, （13.43±4.78）, （15.17±3.82）, （26.39±5.76）, （25.78±4.40）μg·mL^-1, respectively. The antitumor effect of KAL has obvious time-effect and dose-effect dependence. CONCLUSION KAL has significant growth inhibitory effect on B-16, SGC-7901, Colo320, PC-3 and HL60 cells.