乌灵菌粉化学成分研究

Chemical Constituents of Wuling Fermentative Powder

目的对乌灵菌粉的化学成分进行研究。方法采用硅胶柱层析、制备液相等多种分离手段,对乌灵菌粉中石油醚、乙酸乙酯部分进行提取、分离、纯化,通过核磁数据进行结构鉴定。结果分离并鉴定4个化合物,分别为:5-hydroxymellein(1)、5-carboxylmellein(2)、金雀异黄素(3)和5-methylmellein(4)。结论化合物2,3,4为首次从乌灵菌粉提取分离得到;化合物1,2,4为乌灵菌粉的特征性成分。

OBJECTIVE To study the chemical constituents of Wuling fermentative powder. METHODS Four components were extracted, separated and purified from the parts of petroleum and acetic ether by the means of silica gel column chromatography and preparative HPLC methods, and identified by NMR data. RESULTS The four compounds were elucidated as： 5-hydroxymellein（1）, 5-carboxylmellein（2）, genistein（3） and 5-methylmellein（4）. CONCLUSION The later three components are obtained for the first time from Wuling Fermentative powder. 1, 2 and 4 are the characteristic components in Wuling Fermentative powder.