期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

应用环介导等温扩增法进行卡马西平安全用药监测 被引量：2

Monitoring the Safety of Carbamazepine by Loop Mediated Isothermal Amplification
原文传递
导出
摘要 目的建立环介导等温扩增法(loop-mediated isothermal amplification,LAMP)检测卡马西平引起严重皮肤损害的标志基因HLA-B*1502的技术平台。方法对所收集的病例,分别应用LAMP和金标准SSP-PCR进行标志基因HLA-B*1502的检测,验证LAMP方法的准确性。结果 LAMP和SSP-PCR检测38例对照组和2例药疹组患者基因型结果完全一致。结论 LAMP操作简单快捷,且准确可靠,可以在临床上广泛推广,提高卡马西平安全用药水平。 OBJECTIVE To establish a technology platform for testing the marker gene HLA-B＊ 1502 of severe epispasis induced by earbamazepine using the loop-mediated isothermal amplification（LAMP）. METHODS The marker gene HLA-B＊ 1502 was tested by LAMP method and the SSP-PCR method, respectively. And the accuracy of the LAMP method was verified. RESULTS The genotype results of control group and drug eruption group tested with LAMP method were consistent with those using SSP-PCR method. CONCLUSION LAMP method is simple and quick to be operated. It＇s reliable and accurate, and can be widely applied in clinic.
作者 王丽彬 孟玲
机构地区 南京医科大学第一附属医院药剂科
出处 《中国现代应用药学》 CAS CSCD 2014年第5期574-577,共4页 Chinese Journal of Modern Applied Pharmacy
基金 南京市2012年度科技发展计划(药学项目)(2012YX006)
关键词 卡马西平 SJS TEN HLA-B＊1502 环介导等温扩增法 引物序列特异性PCR carbamazepine SJS/TEN HLA-B＊ 1502 LAMP SSP-PCR
分类号 R971.6 [医药卫生—药品]
  • 相关文献

参考文献8

  • 1BAO X P,YANG Y,YANG Z X.Adverse drug reaction of carbamazepine [J].中国误诊学杂志,2009,9(22):5529-5530.
  • 2MAN C B,KWAN P,BAUM L,et al.Association between HLA-B*1502 allele and antiepileptic drug-induced cutaneous reactions in Han Chinese [J].Epilepsia,2007,48(5):1015-1018.
  • 3CHUNG W H,HUNG S I,HONG H S,et al.A marker for Stevens-Johnson syndrome [J].Nature,2004,428(6982):486.
  • 4CHENG S H,KWAN P,NG H K,et al.New testing approach in HLA genotyping helps overcome barriers in effective clinical practice [J].Clin Chem,2009,55(8):1568-1572.
  • 5BUNCE M,ONEILL C M,BARNARDO M C,et al.Phototyping:comprehensive DNA typing for HLA-A,B,C,DRB1,DRB3,DRB4,DRB5 & DQB1 by PCR with 144 primer mixes utilizing sequence-specific primers(PCR-SSP)[J].Tissue Antigens,1995,46(5):355-367.
  • 6何学莲,刘智胜,吴革菲,孙丹,赵培伟,江军,王芳琳,胡家胜,毛冰.卡马西平引起Stevens-Johnson综合征HLA-B*1502基因阴性1例报告并文献复习[J].临床儿科杂志,2012,30(11):1006-1010. 被引量：6
  • 7NOTOMI T,OKAGAMA H,MASUBUCHI H,et al.Loop-mediated isothermal amplification of DNA [J].Nucleic Acids Res,2000,28(12):E63.
  • 8LONJOU C,THOMUS L,BOROT N,et al.A marker for Stevens-Johnson syndrome:ethnicity matters [J].Pharmaco-genomics J,2006,6(4):265-268.

二级参考文献26

  • 1Chung WH, Hung SI, Hong HS, et al. Medical genetics: a marker for Stevens-Johnson syndrome [J]. Nature, 2004, 428(6982): 486.
  • 2Man CB, Kwan P, Baum L, et aL Association between HLA-B*1502 allele and antiepileptic drug-induced cutane- ous reactions in Han Chinese [J]. Epilepsia, 2007, 48(5): 1015-1018.
  • 3Lonjou C, Thomas L, Borot N, etal. A marker for Stevens- Johnson syndrome: ethnicity matters [J]. Pharmacogenom- ics J, 2006, 6(4): 265-268.
  • 4Alfirevic A, Jorgensen AL, Williamson PR, et al. HLA-B locus in Caucasian patients with carbamazepine hypersen- sitivity [J]. Pharmacogenomics, 2006, 7(6): 813-818.
  • 5Locharernkul C, Loplumlert J, Limotai C, et al. Carbam- azepine and phenytoin induced Stevens-Johnson syndrome is associated with HLA-B*1502 allele in Thai population [J]. Epilepsia, 2008, 49(12): 2087-2091.
  • 6Kaniwa N, Saito Y, Aihara M, et al. HLA-B locus in Japa- nese patients with anti-epileptics and allopurinol-related Stevens-Johnson syndrome and toxic epidermal necrolysis [J]. Pharmacogenomics, 2008, 9(11): 1617-1622.
  • 7Chang CC, Too CL, Murad S, et al. Association of HLA- B*1502 allele with carbamazepine-induced toxic epidermal necrolysis and Stevens-Johnson syndrome in the multi- ethnic Malaysian population [J]. Int J Dermatol, 2011, 50(2).. 221-224.
  • 8Mehta TY, Prajapati LM, Mittal B, et al. Association of HLA-B*IS02 allele and carbamazepine-induced Stevens- Johnson syndrome among Indians [J]. Indian J Dermatol Venereol Leprol, 2009, 75(6): 579-582.
  • 9Ozeki T, Mushiroda T, Yowang A, et aL Genome-wide association study identifies HLA-A*3101 allele as a genetic risk factor for carbamazepine-induced cutaneous adverse drug reactions in Japanese population [J]. Hum Mol Gen- et, 2011, 20(5): 1034-1041.
  • 10McCormack M, Alfirevic A, Bourgeois S, et al. HLA- A*3101 and earbamazepine induced hypersensitivity re- actions in Europeans [J]. N Engl J Med, 2011, 364(12): 1134-1143.

共引文献5

同被引文献36

  • 1邢宇俊,吴季荣,徐剑宏,史建荣.环介导等温扩增技术检测转基因组分的研究进展[J].食品安全质量检测学报,2014,5(3):853-860. 被引量：3
  • 2张舒亚,张坤,樊彦莉,薛华杰,印丽萍,陈颖,张璜.食品过敏原羽扇豆成分的环介导等温扩增检测方法[J].食品安全质量检测学报,2014,5(4):1073-1080. 被引量：5
  • 3傅茜,曾文涛,邓素雄,陈连周,王长希.应用PCR-SSP和SSOP对HLA-I类进行基因分型[J].实用医学杂志,2004,20(11):1249-1250. 被引量：1
  • 4NOTOMI T, OKAYAMA H, MASUBUCHI H, et al. Loop- mediated isothermal amplification of DNA J. Nucleic Acids Research, 2000, 28 (12): e63.
  • 5NAGAMINE K, HASE T, NOTOMI T. Accelerated reaction by loop-mediated isothermal amplification using loop primers [J]. Molecular and Cellular Probes, 2002, 16 (3) : 223-229.
  • 6KAWANO S, MAEDA T, WATANABE J, et al. Successful diagnosis of tuberculous lymphadenitis by loop-mediated isothermal amplification of cutaneous samples from an ulcerated surface lesion: a case report [ J ]. Journal of Medical Case Reports, 2014, 8: 254.
  • 7TSAI S S, CHANG Y L, HUANG Y L, et al. Development of a loop-mediated isothermal amplification method for rapid detection of pigeon eircovirus [ J]. Archives of Virology, 2014, 159 (5): 921-926.
  • 8LI C, CHEN J, SHI H, et al. Rapid detection of porcine kobuvirus in feces by reverse transcription loop-mediated isothermal amplification [ J 1. Virology Journal, 2014, 11: 73.
  • 9HASIOW-JAROSZEWSKA B, BUDZSKA D, BORODYNKO N, et al. Rapid detection of genetically diverse tomato black ring virus isolates using reverse transcription loop-mediated isothermal amplification [ J ]. Archives f Virology, 2015, 160 (12): 3075-3078.
  • 10TAO Z Y, ZHOU H Y, XIA H, et al. Adaptation of a visualized loop-mediated isothermal amplification technique for field detection of Plasmodium vivax infection [ J ]. Parasites & Vectors, 2011,4 (1) : 287-290.

引证文献2

二级引证文献7

中国现代应用药学
2014年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部