早期宫颈鳞癌患者血清miR-17的表达及其临床意义

Expression of Serum miR-17 and Its Clinical Significance in Patients with Early-Stage Squamous Cervical Cancer

目的探讨血清microRNA-17(miR-17)诊断早期宫颈鳞癌的价值及其与临床病理特征的关系。方法采集80例宫颈鳞癌患者及20例健康妇女的血清样本,采用实时RT-PCR测定血清miR-17水平,并绘制ROC曲线分析其诊断早期宫颈鳞癌的价值;分析血清miR-17水平与早期宫颈鳞癌临床病理因素的关系。结果早期宫颈鳞癌患者血清miR-17相对表达水平高于正常对照组(P<0.05);ROC曲线下面积0.777,最大约登指数0.5,灵敏度0.95,特异度0.55,对应诊断界值0.858;血清miR-17相对表达水平与早期宫颈鳞癌的临床分期及病理分级有关(P<0.05);而与淋巴结转移、年龄、绝经状况无相关性(P>0.05)。患者肿瘤分化程度越低、临床分期级别增加,血清miR-17水平越高。结论 miR-17在宫颈癌的发生、发展中发挥了作用,对早期宫颈鳞癌的筛查有一定价值。

Objective To evaluate the expression of serum microRNA-17（miR-17） in the diagnosis of early-stage squamous cervical cancer ,and to explore its correlation with clinicopathologic features of the disease .Methods The level of serum miR-17 was detected in 80 patients with squamous cervical cancer and 20 healthy women by real-time RT-PCR,and its diagnostic value was analyzed by receiver operating characteristic curve （ROC curve）.The relationship between the level of serum miR-17 and the clinicopathologic features of the disease was analyzed .Results The relative expression level of serum miR-17 was significantly higher in patients with early-stage squamous cervical cancer in contrast with that in normal controls （P〈0.01）.The area under ROC curve was 0.777 and the max Youden index was 0.5,the sensitivity and specificity were 95%and 55%,respectively ,the cut-off point was 0.858.The relative expression level of miR-17 correlated with clinical stage and pathological grade of early -stage squamous cervical cancer （P〈0.05）, but didn′t correlate with lymph node metastasis ,the patients′age and menopausal status （P〉0.05）.Lower degree of tumor differentiation ,higher clinical stage and higher level of serum miR-17.Conclusion miR-17 plays a role in the occurrence and development of squamous cervical cancer ,and it is valuable for the screening of early-stage squamous cervical cancer .