牡公灌肠散提取纯化工艺考察

Study on the extraction and purification process of Herba Taraxaci and Ostreae concha enema powders

目的:优选牡公散中蒲公英与煅牡蛎合提的最佳提取纯化工艺。方法:以浸膏得率及蒲公英中的有效抗菌成分咖啡酸的量为评价指标,采用正交试验法优选最佳提取工艺;以浸膏得率、咖啡酸的量为评价指标,采用单因素法,比较醇沉和壳聚糖纯化工艺,优选最佳纯化工艺。结果:最佳提取纯化工艺为加8倍水浸泡1.5 h,提取3次,每次1 h,将提取液浓缩至药液比为1∶2(g·ml-1),醇沉至含醇量为80%,4℃静置24 h。结论:该工艺简便可行、稳定性好,可为牡公散制剂工艺提供可靠的依据。

OBJECTIVE To study the optimum extraction and purification process of HerbaTaraxacum and calcined oyster. METHODS In the orthogonal design test, the yield of extract and the weight of coffeic acid which was one of the effective an- tibacterial components in HerbaTarazacum were used as evaluation indexes to determine the optimum extraction process. In the single factor test, the same indexes mentioned above were also used to indicate the optimum purification process between al- cohol precipitation and chitosan clarification. RESULTS The optimum extraction and purification process was determined as ex-tracting 3 times and 1 h for each time with 8 fold of water （v/w, soaking 1.5 h before the first extraction）, and the concentra- tion ratio between medicinal materials and water was 1：2 （g·ml-1 ）. After alcohol precipitation at a concentration of 80% etha- nol, deposited for 24 h at 4 ℃. CONCLUSION This process is convenient, feasible and stable to provide reliable basis for the enema powders.