积雪草多糖的脱脂工艺研究

Study on degreasing process of polysaccharides from entella asiatica

为优选并确立积雪草的最佳脱脂工艺,考察积雪草不同脱脂溶剂对多糖得率和脱脂率的影响,通过单因素试验和正交试验对脱脂工艺进行优化.最佳脱脂工艺为:料液比(g/mL)1︰10,乙醚-乙醇溶液(V乙醚︰V乙醇=2︰3)在35℃下脱脂6 h.在此脱脂方案下,积雪草多糖平均得率为2.69%(n=5),RSD为2.23%,脱脂率为15.87%(n=5).该脱脂方案稳定,可使多糖得率更高.

Objective： To study the optimization of degreasing conditions on polysaccharides from Centella asiatica. Method： The effect of different degreasing agents on polysaccharide yield of Centella asiatica ？and ratio？of degreasing was studied. The optimal conditions of degreasing conditions of Centella asiatica were studied through single-factor and orthogonal experiments. Result： The optimal degreasing conditions are as follows： the ratio of material to solvent was 1：10, the degreasing agent was ether and alcohol （v： v=2：3）, and the degreasing time was 6 hour at 35℃. Under these conditions, the yield of polysaccharide in Centella asiatica was 2.69% （n=5）, RSD was 2.23%, the ratio of degreasing was 15.87% （n=5）. Conclusion： The degreasing conditions of polysaccharides in Centella asiatica was stable and efficient.