聚合酶链反应检测尖锐湿疣患者人乳头瘤病毒的结果分析

THE ANALYSIS OF THE RESULTS OF POLYMERASE CHAIN REACTION TO TEST THE HUMAN PAPILLOMA VIRUS OF THE PATIENTS SUFFERING FROM CONDYLOMA ACUMINATUM.

目的 :探讨聚合酶链反应 (PCR)在检测尖锐湿疣病人中人乳头瘤病毒的应用。方法 :采用 PCR的方法。结果 :2 0 3例被临床医师诊断或怀疑为尖锐湿疣 (CA)患者的标本进行了人乳头瘤病毒 DNA (HPV - DNA)的检测。其中 6 8例为皮损组织块标本 ,此部分患者的 CA症状典型 ,被临床医师诊断为 CA,6 8例均被检测出 HPV- DNA,检出率 10 0 % ;135例为生殖器分泌物标本 ,此部分患者的 CA症状还不典型 ,仅为临床医师怀疑对象 ,从中检测出 HPV- DNA 10 9例 ,检出率 80 .7%。结论 :用 PCR检测标本的 HPV- DNA,可对 CA患者进行准确地早期诊断 ,从而有利于患者的早期发现和治疗。

Objective:To explore the application of PCR to examine HPV-DNA of CA.Methods:Using polymerase chain reaction(PCR).Results:We tested human papilloma virus DNA(HPV-DNA) in 203 samples of the patients whom clinicians diagnosed or suspected suffering from condyloma acurninatum(CA).Among them,68 samples were of skin organization damage.These patients had typical symptoms of CA and were diagnosed as CA cases by doctors.They were all detected to have HPV-DNA.The positive rate was 100%;135 samples were of reproductive organs secretion and these patients didnt have typical CA symptoms.They were only suspected as CA cases by doctors,among them,109 samples were detected to have HPV-DNA.The positive rate was 80 7%.Conclusion:The above results indicate that using PCR to test HPV-DNA is an accurate early stage of diagnosis for the CA cases,and therefore is favorable for the early discovery and treatment of CA cases.