副猪嗜血杆菌的分离与血清型鉴定

Identification and Serological Investigation on Haemophilus Parasuis

为了解副猪嗜血杆菌的血清型流行情况,采用细菌分离培养结合PCR的方法,对2008年至2012年,从山东、河南、河北、湖南、江苏、上海的多个猪场送检的186份疑似样品进行副猪嗜血杆菌的检测,再通过琼脂扩散试验对分离菌株进行了血清型鉴定。结果表明,从186份疑似样品中最终分离鉴定到57株副猪嗜血杆菌,分离率为30.6%。结果提示,我国副猪嗜血杆菌病存在不同程度的感染,以血清4型和5型最为流行,其次是血清1、12、13型,且该病的发生与季节和猪的日龄有关。

To investigate the serological of HPS,186 suspect samples from different provinces were examined between 2008 and 2012 for Haemophilus parasuis （HPS） by isolation and PCR,and the serotypes of isolated strains were thereafter classified by the agar diffusion test.57 strains had been isolated and identified with the isolating rate up to 30.6 ％.The result showed that there were different levels of infection of HPS in China,with HPS serotype 4 and 5 being the most dominant ones followed by serotype 1,12 and 13.The close relation between the epidemics of HPS,seasons and the age of piglets were also observed.