摘要
目的:观察转化生长因子β_1(transforming growth factor-β_1,TGF-β_1)对培养人眼小梁细胞分泌Ⅳ型胶原的影响。方法:在成功地培养人眼小梁细胞的基础上,应用酶联免疫吸附实验(enzyme-linked immunosorbentassay ELISA)及免疫组织化学技术,分别观察1ng/ml TGF-β1作用于小梁细胞后培养上清液中及细胞铺片上Ⅳ型胶原的含量。结果:ELISA法:TGF-β_1作用第一个4天,实验组Ⅳ型胶原的含量为187.50±29.01pg/ml(x±s),对照组为93.75±20.56pg/ml,两者间的差异有统计学意义。TGF-β_1作用第二个4天,实验组为128.75±52.02pg/ml,对照组为91.25±21.36pg/ml,差异无统计学意义。Ⅳ型胶原的含量在对照组之间及实验组之间也无统计学差异。免疫组化法:TGF-β_1作用12天,实验组铺片与对照组相比Ⅳ型胶原的阳性着色增强。结论:TGF-β_1可以促进培养的人眼小梁细胞分泌Ⅳ型胶原,它可能是原发性开角型青光眼患者小梁细胞外基质堆积的原因之一。眼科学报2000;16:217～219。
Purpose: To investigate the effect of transforming growth factor-β1 (TGF-β1) on the expression of collagen type IV in cultured human trabecular cells.
Methods: On the basis of successful culture of human trabecular cells, we detected the content of collagen type IV in the media and covers of trabecular cells, treated with TGF-β1 (lng/ml), by using enzyme-linked immunosorbent assay(ELISA) and immuno-histochemical techniques respectively.
Results: ELISA method demonstrated that, in the first four days, there was statistically significant difference between TGF-β1 treated and control groups with the collagen type IV content of 187. 50±29. 01pg/ml and 93. 75±20. 50pg/ml respectively, then in the second four days, there was no statistically significant difference between experimental groupof128. 75±2. 02pg/mlandcontrolgroupof91. 25±?1. 36pg/ml. Neither statistical difference was found between two treated groups, nor was between two control groups. Immunohistochemical technicques showed, the positive staining of collagen type IV was stronger in cell covers of experimental group treated with TGF-β1 for 12 days than that of control group. Conclusion: TGF-β1 can increase the expression of collagen type IV in cultured trabecu- lar cells, it may be among causes of abnormal accumulation of extracellular matrices in trabecular meshwork of eyes with primary open angle glaucoma. Eye Science 2000; 16: 217- 219.
出处
《眼科学报》
2000年第4期217-219,共3页
Eye Science
