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狗胆囊上皮细胞的分离及原代培养 被引量:2

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摘要 胆囊的主要功能是贮存、浓缩和排泄胆汁,上述功能的发挥与胆囊上皮细胞有着十分密切的联系[1-6].胆囊好发疾病如胆囊炎、胆石症、胆囊癌等,都是以胆囊上皮细胞为病变的靶位、损伤因素攻击的中心 [7,8 ].可以认为,胆囊上皮细胞是胆囊生理功能及病理状态的中心环节[9].而要研究胆囊上皮细胞的粘液分泌能力、离子转运功能、对炎症递质的反应和病理状态改变等,首先需进行胆囊上皮细胞的分离和原代培养.由于正常人胆囊标本来源困难,狗和兔被作为胆囊上皮细胞分离培养的常用动物.我们经过反复摸索,在国内首次成功地原代分离培养了狗胆囊上皮细胞,为进一步研究胆囊的生理功能和相关疾病奠定了基础.
作者 刘苹 陈东风
出处 《世界华人消化杂志》 CAS 2001年第1期99-100,共2页 World Chinese Journal of Digestology
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