微泵恒速灌注叠氮钠对大鼠学习记忆的影响

Chronic infusion of sodium azide by minipump induces deficit of learning memory ability in rats

目的 利用线粒体细胞色素C氧化酶抑制剂叠氮钠 ,观察其对模型大鼠学习记忆能力的影响。方法 SD大鼠皮下埋植Alzet微泵 ,连续恒速给予叠氮钠共 30天 ,给药 1mg·kg-1·h-1和 2mg·kg-1·h-1两个剂量组。Morris水迷宫、避暗实验检测动物学习记忆能力 ,旷场分析检测空间探索能力及兴奋性 ,攀网实验检测肌力。结果 微泵恒速灌注叠氮钠导致模型大鼠水迷宫游出时间及距离延长、错误次数增加 ,避暗潜伏期缩短、错误次数增加 ,旷场分析中对外界的空间探索能力、适应性及兴奋性均降低 ,但肌力无明显异常。结论 线粒体细胞色素C氧化酶活性下降可以导致动物学习记忆能力下降 ,微泵恒速灌注叠氮钠大鼠可作为一种拟痴呆模型 。

Objective Because of the proposed importance of mitochondrial cytochrome c oxidase (COX) decrease in Alzheimer's disease, the inhibitor of COX(sodium azide) was used to observe the influence of mitochondrial deficiency on learning memory ability of rats. Methods Rats were administrated with azide at 1mg·kg -1 ·h -1 or 2mg·kg -1 ·h -1 subcutaneously via a Alzet minipump for 30days. Then learning memory ability was determined by the Morris watermaze and passive avoidance tests. Spontaneous motor activity was investigated to show the exploration and excitability of rats. Results Drugged rats showed learning memory deficit and decrease in spontaneous motor activity. However, it didn't show any abnormality in horizontal and vertical motor activity. Conclusion The results of present study indicate COX deficiency can induce decline of learning memory and exploration ability in rats. Chronic Infusion of sodium azide via minipump may serve as a tool for developing the experimental model of dementia.