吗啡依赖大鼠模型的戒断行为学比较

Behavioral comparation on withdrawal syndromes in morphine dependent rats

目的 研究吗啡依赖大鼠模型建立方式和催促戒断给药剂量对戒断强度的影响及其相互关系。方法 对两种常用吗啡依赖大鼠模型在不同剂量纳洛酮催促戒断后 ,观察戒断体征及体重减轻来评估戒断强度。结果 两组方法均成功建立了大鼠吗啡依赖。 5日法 (吗啡总剂量 380mg·kg-1)模型大鼠在 2mg·kg-1和 4mg·kg-1纳洛酮催促剂量下戒断强度无显著性差异 (P >0 .0 5 )。 12日法模型 (吗啡总剂量 136 5mg·kg-1)戒断强度随纳洛酮剂量增加显著上升 (P <0 .0 1) ,但仅在 4mg·kg-1纳洛酮催促剂量时显著高于 5日法模型。结论 应根据不同的实验要求 ,选择适当的吗啡依赖模型的建立方法 。

Objective To investigate the the effect and interaction of morphine dependence model and naloxone doses on withdrawal intensity. Methods To observe the withdrawal signs and weight loss in two kinds of morphine dependent rats after the precipitating by naloxone of different doses an devaluate the withdrawal intensity.Results Morphine dependence were successfully induced in two ways.There was no significant difference of withdrawal intensity in five day model(total morphine dose 380mg·kg -1 ) after precipitating by naloxone of 2mg·kg -1 and 4mg·kg -1 (P>0.05). Withdrawal intensity of twelve day model(total morphine dose 1365mg·kg -1 )increased significantly with the increase of naloxone dose(P<0.01),but it was more intensive significantly than five day model only after 4mg·kg -1 dose of naloxone. Conclusion Appropriate method should be used to induce morphine dependence and these experimental factors should be combined in assessment of withdrawal intensity.