洗涤式自体血回输中不同温度洗涤液对人红细胞形态结构的影响

Effect of different temperature scrubbing solution on red blood cell morphological structure

目的探讨自体血回输中不同温度洗涤液对人红细胞形态结构的影响。方法选择临床自体血回输者60例,根据洗涤液NS温度的不同,分为三组[4℃、24℃(常温)、37℃]分别记录洗涤前后的回收血液pH值、Na+、K+、血红蛋白(Hb)、红细胞压积(Hct),收集回输血液中的红细胞,分别在电子显微镜下观察红细胞的形态变化。结果洗涤后三组Hb、Hct、血钠水平均较洗涤前显著升高(P<0.01),24℃组和37℃组Hb、Hct升高幅度并不如4℃组(P<0.05)。三组洗涤后血K+浓度较洗涤前显著下降(P<0.01)。洗涤后三组并没有出现显著红细胞变形,随着时间的延长,三组棘形红细胞均有所增加(P<0.01),而温度越高,随着时间的延长,棘形红细胞比例越高(P<0.01)。结论自体血回输过程中,4℃的洗涤液对红细胞形态的维持优于常温和37℃,尤其随着时间的延长,优势更加明显。

Objective To discuss effect of different temperature scrubbing solution on red blood cell morphological structure. Methods Selected 60 cases with autologous blood transfusion were divided into 4℃ group, 24℃ group and 37℃ group. pH, Na＋,K＋,Hb, Hct were detected before being scrubbed and after being scrubbed. Red blood cell struc-ture after being scrubbed were observed. Results Levels of Hb, Hct, Na＋ of three groups after being scrubbed were higher than those before being scrubbed（P〈0.01）. And group 4℃was higher than group 24℃and group 37℃（P〈0.05）. Level of K＋of three groups decreased after being scrubbed （P〈0.01）. After being scrubbed, spines shape of red blood cells increased with extension of time （P〈0.01）. With increasing of temperature, spines shape of red blood cells in-creased （P〈0.01）. Conclusion In the process of autologous blood doping, scrubbing solution of 4℃for red blood cell morphology keeping is better than 24℃and 37℃.