二溴二甲基乙内酰脲的消毒杀菌作用

Laboratory observation on disinfection by 2,4-dibromo-5,5-dimethylhydantoin

目的 探索二溴二甲基乙内酰脲对细菌繁殖体、枯草杆菌黑色变种芽胞的消毒杀菌作用及对 HBs Ag抗原性破坏作用。方法 中和剂选择试验、定量杀菌试验、HBs Ag破坏试验。结果 以含有效溴 4mg· L- 1 和 2 mg· L- 1 浓度的二溴二甲基乙内酰脲分别作用 10 m in和 2 0 m in,对金黄色葡萄球菌和大肠杆菌杀灭率达 10 0 % ,以含有效溴 2 0 0 0 m g· L- 1 浓度作用 30 m in,对枯草杆菌黑色变种芽胞的杀灭率达 10 0 % ;以含有效溴 10 0 0 m g· L- 1 浓度作用 5 m in,可将悬液中 HBs Ag的抗原性完全破坏。结论 二溴二甲基乙内酰脲作为释放活性溴的消毒杀菌剂 ,对大肠杆菌、金黄色葡萄球菌、枯草杆菌黑色变种芽胞有良好杀灭作用 ,并可使 HBs

Objective To study efficacy of 2,4 dibromo 5,5 dimethylhydantoin in killing vegetative forms of bacteria and spores of B. subtilis var.niger, and efficacy of 2,4 dibromo 5,5 dimethylhydantoin in destroying antigenicity of HBsAg. Methods Neutralizer test and efficacy of solution of 2,4 dibromo 5,5 dimethylhydantoin in killing vegetative forms of bacteria and spores. Neutralizer test and efficacy of solution of 2,4 dibromo 5,5 dimethylhydantoin in destroying HBsAg antigenicity in suspension. Results The killing rate of Staphylococcus aureus and E. coli. was 100% after exposure to its solution containing 4 mg·L -1 and 2 mg·L -1 available bromo after 10 min and 20 min. The killing rate of spores of Bacillus subtilis var. Niger also was 100% after exposure to its solution containing 2 000 mg·L -1 available bromo after 30 min. Its solution containing 1 000 mg·L -1 available bromo with could destroy HBsAg in suspension for 5 min. Conclusions 2,4 dibromo 5,5 dimethylhydantoin can effectively kill vegetative forms of bacteria and spores of B. subtilis var.niger, and can completely destroy the antigenicity of HBsAg in the water.