高效液相色谱法测定牛樟芝中腺苷的含量

High Performance Liquid Chromatography in Determination of Adenosine Content in Antrodia Camphorata

目的：采用高效液相色谱法测定牛樟芝中腺苷的含量。方法色谱柱：Agilent Zorbax SB-C18柱（4.6 mm ×250 mm,5μm）,流动相：乙腈-0.01 mol/L磷酸二氢钾溶液（6∶94）,流速：1.0 ml/min,检测波长：260 nm,柱温：30℃,进样量：10μl。结果腺苷在2.696~67.40 g/ml范围内与其峰面积呈现良好的线性关系（r=0.9999）,平均回收率为99.89%,相对标准偏差为1.71%（n=6）。结论该方法简便准确,灵敏度高,重复性好,为控制牛樟芝的质量提供了科学依据。

Objective To detect the Adenosine content in Antrodia Camphorata using high performance liquid chromatography （HPLC）. Methods The chromatographic column was Agilent Zorbax SB-C18 column （4. 6 mm × 250 mm, 5 μm）; the mobile phase was acetonitrile-0. 01 mol＆#183;L-1 KH2PO4 solution （6∶ 94）; the flow rate was 1. 0 ml/min-1; the detection wavelength was 260 nm;the column temperature was set at 30℃;the injection volume was 10 μl. Results A good linear relationship between the peak area and Adenosine concentration was observed within the range of 2. 696-67. 40 mg/ml （r=0. 9999）. The average recovery was 99. 89% with 1. 71% of relative standard devia-tion （n=6）. Conclusion This method is accurate and has good sensitivity and reproducibility, and can provide scien-tific basis for controlling the quality of Antrodia camphorata.