导流杂交基因芯片技术在女性生殖道人乳头状瘤病毒检测中的应用价值

Application Value of Flow-Through Hybridization with GeneChip Technique in Detection of the Human Papilloma Virus of Female Genital Tract

目的：评价导流杂交基因芯片技术在女性生殖道人乳头状瘤病毒（ HPV）感染检测中的应用价值,探讨女性生殖道感染最常见的HPV基因型及好发年龄。方法采用导流杂交基因芯片技术对545例患者的宫颈细胞学标本进行宫颈细胞21种HPV-DNA基因分型检测。比较细胞学正常组（n=120）、细胞学异常组（n=425）及细胞学异常各类型[宫颈慢性炎症174例、宫颈上皮内瘤变（ CIN）Ⅰ级102例、CIN Ⅱ级74例、CIN Ⅲ级36例、宫颈癌16例和尖锐湿疣23例]组HPV阳性率。将细胞学异常组分为4个年龄组：20~岁组85例、31~岁组140例、41~岁组90例、51~岁组110例,比较各年龄组HPV阳性率。结果细胞学正常组HPV阳性率为22.5%（27/120）,细胞学异常组为87.3%（371/425）。细胞学异常组及细胞学异常类型6组HPV阳性率分别与细胞学正常组比较,差异均有统计学意义（P〈0．01）。细胞学正常组中6种最为常见的HPV基因型按阳性率递减依次为HPV16、68、18、52、58、11；细胞学异常组中6种最常见的HPV基因型依次为HPV 16、52、58、18、33、31。31~岁组、41~岁组HPV阳性率显著高于51~岁组（P〈0．01）,31~岁组HPV阳性率最高,41岁后随年龄的增长逐渐降低。结论采用导流杂交基因芯片技术一次试验能联合检测出多种HPV亚型感染及型别分布,从而提高由HPV感染引起的妇科肿瘤的防治水平。

Objective To evaluate the clinical value of flow-through hybridization with genechip technology in detection of human papilloma virus （ HPV） of female genital tract, and to explore the most common HPV genotypes and high nosogenic age. Methods The cervical cells of 21 kinds of HPV genotyping assays were performed for 545 cervical cytology specimens using flow-through hybridization with genechip technology. The HPV-positive rates were compared a-mong normal cytology group （ n=120 ） , abnormal cytology group （ n=425 ） and abnormal types of abnormal cytology group 174 cases of chronic cervical inflammation, cervical intraepithelial neoplasia （102 cases of CIN I, 74 cases of CIN II and 36 cases of CIN III） , 16 cases of cervical carcinoma and 23 cases of condyloma acuminatum] . Abnormal cytology group was divided into 4 age subgroups：85 cases of the 20-31 age group, 140 cases of the 31-41 age group, 90 cases of the 41-51 age group and 110 case of older than 51 age group, and HPV infection rates in the four groups were com-pared. Results The HPV-positive rate was 22. 5% （27/120） in normal cytology group and 87. 3% （371/425） in ab-normal cytology group respectively. The differences in HPV-positive rates in abnormal cytology group and 6 subgroups in abnormal types of abnormal cytology group respectively compared with that in normal cytology group were statistically sig-nificant （P〈0. 01）. Six of the common HPV genotypes in normal cytology group were HPV16, 68, 18, 52, 58 and 11 in turn （by diminishing positive rate）;six of the most common HPV genotypes in abnormal cytology group were HPV 16, 52, 58, 18, 33, 31. The HPV-positive rates in the 31-41 age and the 41-51 age groups were significantly higher than that in older than 51 age group （P〈0. 01）, the 31-41 age group had the highest HPV-positive rate, and the rate gradu-ally decreased with age increasing when the patients were 41 years old. Conclusion The flow-through hybridization with＆amp;nbsp;genechip technology may detect a variety of HPV infected subtypes and group types in just one test to improve the level of prevention and treatment of gynecologic cancer induced by HPV infection.